相关实验视频

Updated: Jul 2, 2025

Preparation of Virus-Enriched Inoculum for Oral Infection of Honey Bees Apis mellifera

Preparation of Virus-Enriched Inoculum for Oral Infection of Honey Bees Apis mellifera

Published on: August 26, 2020

哺乳蜜蜂的行为和与感官相关的基因因被变形翼病毒变体A改变

Silva Diego¹, Arismendi Nolberto², Alveal Juan Pablo³

¹Laboratorios de Virología y Patologías en Abejas, Facultad de Agronomía, Universidad de Concepción, Av. Vicente Méndez 595, Chillán 3780000, Chile.

|February 23, 2024

概括

蜜蜂的变形翼病毒 (DWV) 感染显著损害了嗅觉反应,并减少了母蜂的关键基因表达. 这种病毒影响扰乱了感官处理,影响了蜂群行为和凝聚力.

科学领域:

昆虫学昆虫学是一门学科.
病毒学病毒学
神经科学是一个神经科学.

背景情况:

昆虫的行为,特别是嗅觉,依赖于气味结合蛋白 (OBPs) 进行环境识别.
蜜蜂的行为容易受到病原体的影响,如变形翼病毒 (DWV),DWV-A影响嗅觉敏感性和OBP基因表达.
蜜蜂大脑中的DWV复制进一步损害了感官信息处理.

研究的目的:

为了评估护士蜜蜂在感染DWV-A时对费洛蒙的行为反应.
分析DWV-A感染的护士蜜蜂中前和后突触神经元基因 (神经素-1,神经-1) 和OBP (OBP5,OBP11) 的基因表达的变化.

主要方法:

护士蜜蜂被接种了DWV-A.
对醇 (幼虫激素) 的行为反应使用Y管嗅计进行了评估.
使用qPCR量化病毒载量,OBP基因表达 (OBP5,OBP11) 和神经元基因表达 (神经素-1,神经-1).

主要成果:

高DWV-A病毒载荷显著降低了护士蜜蜂 (10日和15天大) 正确识别费罗蒙的能力.
在高度感染DWV-A的护士蜜蜂中,OBP5,OBP11,neuroxin-1和neurogilin-1的基因表达减少.

结论:

在蜜蜂中,DWV-A感染会破坏传感信息处理.
由于DWV-A感染而减少的OBP和神经元基因表达,可能导致护士蜜蜂活动减少并影响蜂群凝聚力.

关键词:

这是一个DWV-A.在OBPs中,OBPs是指操作系统.护士蜜蜂是什么样的蜜蜂嗅觉反应嗅觉反应突触基因是突触基因中的一个.

更多相关视频

RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees Apis mellifera

RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees Apis mellifera

Published on: July 25, 2013

SwarmSight: Real-time Tracking of Insect Antenna Movements and Proboscis Extension Reflex Using a Common Preparation and Conventional Hardware

SwarmSight: Real-time Tracking of Insect Antenna Movements and Proboscis Extension Reflex Using a Common Preparation and Conventional Hardware

Published on: December 25, 2017

相关概念视频

Frequency-dependent Selection

Frequency-dependent Selection

When the fitness of a trait is influenced by how common it is (i.e., its frequency) relative to different traits within a population, this is referred to as frequency-dependent selection. Frequency-dependent selection may occur between species or within a single species. This type of selection can either be positive—with more common phenotypes having higher fitness—or negative, with rarer phenotypes conferring increased fitness.