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子副产品的膜分离水解剂用于提高生物活性的度

Janka Dibdiakova¹, Josipa Matic², Sileshi Gizachew Wubshet²

¹Aquateam COWI AS, Karvesvingen 2, 0579 Oslo, Norway.

|February 23, 2024

概括

副产品水解酸的超过 (UF) 和微过 (MF) 有效地保留了双基酶IV (DPP-IV) 抑制. UF是缩生物活性的最佳,显示出显著的健康益处潜力.

科学领域:

生物技术和食品科学生物技术和食品科学
分离科学和膜技术分离科学和膜技术

背景情况:

膜工艺对于分离和净化生物技术产品至关重要.
的副产品水解剂含有具有潜在健康益处的生物活性.
双基酶IV (DPP-IV) 抑制是代谢健康的一个关键目标.

研究的目的:

用膜工艺研究从副产品中分离生物活性的水解剂.
评估微过 (MF),超过 (UF),纳米过 (NF) 和反透 (RO) 对DPP-IV抑制活性的影响.
为了将的分子量与DPP-IV抑制活性相关联.

主要方法:

副产品的酶化水解,其次是MF,UF,NF和RO.
测定水解剂和透剂的DPP-IV抑制活性.
用有机碳和检测 (SEC-OCD-OND) 进行大小排除色谱,用于分子量分析.

主要成果:

MF和UF处理产生了具有最高DPP-IV抑制率的化物 (59.5-60.0%在1 mg/mL).
NF显著降低了抑制活性,在度中保留了生物活性.
化物透物显示出强大的DPP-IV抑制 (IC50 = 0.75 mg/mL),平均分子量为883g/mol.

结论:

超过 (UF) 是一种有效的技术,用于从副产品水解物中缩小分子量生物活性.
鉴定的具有显著的DPP-IV抑制活性,与已知的抑制剂相当.
这些发现凸显了来自的生物活性的食品和医疗应用潜力.

关键词:

这是LC-OCD.生物活性类的生物活性.酶性蛋白质水解是一种酶性蛋白质水解.膜过器的过方式是净化净化净化净化

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