基质特性对细胞行为和纳米颗粒吸收在人类纤维细胞和上皮细胞的影响
Mauro Sousa de Almeida1, Aaron Lee1,2, Fabian Itel3
1Adolphe Merkle Institute and National Center of Competence in Research Bio-Inspired Materials, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland.
Nanomaterials (Basel, Switzerland)
|February 23, 2024
概括
细胞外基质 (ECM) 显著影响纳米粒子 (NP) 与细胞的相互作用. 基质的刚性和对齐影响细胞行为和NP吸收,这对纳米医学发展至关重要.
科学领域:
- 生物材料科学 生物材料科学
- 细胞生物学 细胞生物学
- 纳米技术 纳米技术
背景情况:
- 纳米医学提供治疗前景,但纳米粒子 (NP) 与细胞的相互作用尚不清楚.
- 纤维化和癌症等疾病中的细胞外基质 (ECM) 变化可能会影响细胞-NP动态.
- 了解细胞-ECM相互作用对于有效的纳米药物交付至关重要.
研究的目的:
- 研究基质物理特性如何模仿ECM,影响细胞行为和NP吸收.
- 为了比较不同基质上的肺上皮细胞和纤维细胞中NP的吸收.
- 确定细胞矩阵相互作用在纳米医学向中的作用.
主要方法:
- 使用电制造对齐和非对齐的软聚氨 (PU) 纳米纤维支架.
- 在PU支架和组织培养塑料 (TCP) 上培养A549肺上皮细胞和MRC-5肺纤维细胞.
- 评估细胞附着,生长,细胞因子释放和二氧化NP吸收.
主要成果:
- 这两种细胞类型都在PU支架上坚持并生长,没有细胞毒性,但与TCP相比,细胞因子释放增加.
- 与TCP相比,纤维细胞在PU支架上的二氧化NP吸收量增加了三倍以上.
- 表皮细胞的NP吸收不受基质类型或对齐的显著影响.
结论:
- 细胞矩阵相互作用取决于基质的机械特性和细胞类型.
- ECM特征极大地影响了纳米粒子吸收.
- 设计有效的纳米药物需要考虑组织机械性质,以便有针对性的输送.
相关概念视频
01:42The Extracellular Matrix
Overview
01:29The Extracellular Matrix
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
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00:55Microvilli
Microvilli are tiny finger-like projections found on the surface of certain cells. Their purpose is to increase the surface area of the cell's apical surface, resulting in more effective absorption or secretion of substances.
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These microvilli are predominantly present in cells lining the small intestine, kidney tubules, and certain cells in the respiratory and reproductive systems. By significantly expanding the surface area of the cell membrane, microvilli enhance the cell's capacity to...