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使用红外光谱学,单变量统计学和线性差异分析 (LDA) 来快速分类患有帕拉科西多伊多米症的患者的血清

Alessandra Koehler¹, Maria Lúcia Scroferneker^1,2, Nikolas Mateus Pereira de Souza³

¹Postgraduate Program of Medicine: Medical Sciences, Universidade Federal do Rio Grande do Sul-UFRGS, Porto Alegre 90035-003, Brazil.

Journal of fungi (Basel, Switzerland)

|February 23, 2024

概括

福里埃变换红外光谱 (FTIR) 提供了一种新的,高度准确的诊断工具,用于帕拉科西迪奥多米科斯 (PCM). 这种方法显示出超越当前诊断技术的潜力,用于这种被忽视的热带真菌菌.

科学领域:

医学真菌学医学真菌学
频谱学是一种光谱学.
生物化学生化学

背景情况:

甲状腺菌 (PCM) 是一种具有诊断限制的全身性真菌感染.
目前PCM的诊断方法包括真菌可视化和血清学技术,每个都有固有的缺点.

研究的目的:

开发和评估使用福里埃变换红外光谱 (FTIR) 的PCM新型诊断工具.
评估FTIR光谱学的准确性和潜力,作为PCM的快速和可靠的诊断方法.

主要方法:

使用减弱总反射 (ATR) FTIR光谱分析了224个血清样本 (132名PCM患者,92名对照者).
统计分析包括t测试,接收器运行特征 (ROC) 曲线,主要组件分析 (PCA) 和线性差异分析 (LDA).

主要成果:

通过FTIR光谱检测发现了PCM患者和对照组之间的光谱差异.
在1551-1095厘米-1区域的ROC曲线分析产生了高的诊断潜力,具有特定的波数 (1264厘米-1),达到97.73%的灵敏度和94.22%的准确度.
在PCA和LDA中,使用精选的光谱变量证明了完全分离组和100%的诊断准确性.

结论:

FTIR光谱法为PCM诊断提供了一种简单,快速和高度准确的方法.
拟议的基于FTIR的方法与PCM的现有诊断方法相比,显示出更高的准确性.
这种技术对改善偏菌菌的诊断具有显著的前景,这是一个被忽视的热带菌.

关键词:

里埃变换红外光谱法红外光谱法在ROC曲线上,ROC曲线线性差异分析线性差异分析帕拉科科西迪奥伊多米科西斯摄影诊断摄影诊断摄影诊断系统性真菌菌的发生.

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