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在海洋红色微藻中重建长链多不和酸合成途径 Porphyridium cruentum 使用脂管学和转录学
Tao Li1, Chulin Li1, Weinan Wang1
1CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Institution of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.
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概括
海洋微藻Porphyridium产生乙酸 (EPA) 和酸 (ARA). 这项研究表明,EPA主要通过omega-6途径合成,ARA可能在叶绿体中转化为EPA.
科学领域:
- 海洋生物技术 海洋生物技术
- 微藻类脂质代谢 微藻类脂质代谢
- 多不和脂肪酸合成方法
背景情况:
- 海洋红色微藻 (Porphyridium) 合成有价值的长链多不和脂肪酸 (LC-PUFA),如乙酸 (EPA) 和酸 (ARA).
- 在Porphyridium中EPA和ARA的精确分布和生物合成途径仍然不完全理解,阻碍了有针对性的生产策略.
研究的目的:
- 为了阐明EPA和ARA在* Porphyridium cruentum* CCALA 415中的分布和合成途径.
- 调查限对脂肪酸概况和与PUFA合成相关的基因表达的影响.
主要方法:
- 在充满和有限的条件下种植 Porphyridium cruentum.
- 综合分析包括脂肪酸含量确定,转录组学和脂质组学.
- 甘油脂分子物种的识别和脂肪酸成分的量化.
主要成果:
- 的限制促进了储存脂质和omega-6脂肪酸的合成,同时减少了膜脂质和omega-3脂肪酸的含量.
- 阿拉基酸 (ARA) 主要存在于酸丁 (PC),而乙酸 (EPA) 主要局部存在于单酸丁糖化物 (MGDG).
- 基因表达分析表明脂肪酸脱酶 (FADs) 的显著调节,主要转向用于EPA合成的omega-6通路利用.
结论:
- 在P. cruentum中,大多数EPA通过omega-6路径进行合成,提出的机制涉及ARA运输和在质体中的转化.
- 的可用性极大地影响脂质组成和PUFA概况,为优化特定脂肪酸的微藻种植提供了潜力.
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