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了解气可用性的影响:在微藻种植中提高富可桑生产力的限制因素

To Quyen Truong^1,2, Yun Ji Park², Jessica Winarto^1,3

¹Division of Bio-Medical Science & Technology, Korea Institute of Science and Technology (KIST) School, University of Science and Technology, Seoul 02792, Republic of Korea.

|February 23, 2024

概括

高的条件通过增强黄素a/c结合蛋白 (FCP) 的形成,促进微藻中的黄素 (FX) 生产. 将低光和高相结合,最大限度地提高了工业应用的FX产量.

科学领域:

* * 海洋生物技术
* 微藻种植的方法
* 光合作用色素生物合成

背景情况:

* 的可用性对于微藻生长和颜料生产至关重要.
* 在不同含量下调节富可桑丁 (FX) 生物合成的精确机制尚未完全理解.
*优化Phaeodactylum tricornutum中的FX生产具有重要的生物技术潜力.

研究的目的:

* 为了研究不同度对Phaeodactylum tricornutum*的FX生物合成的影响.
* 通过的可用性来阐明FX调节的基础分子机制.
*通过控制的种植策略,优化外汇生产率.

主要方法:

* 在正常,有限和高初始度下进行 *P. tricornutum* 的批量培养.
*分析微藻生长和光合作用色素的产生.
*对富可桑丁-甲a/c结合蛋白 (FCP) 和关键生物合成基因的蛋白质和转录基因分析.

主要成果:

*高 (HN) 条件通过延长生长阶段和增加生物质来促进外汇生产率.
* 在HN条件下观察到胡卜素生成的升级,特别是FCP的形成.
* 关键的FCP蛋白质 (LHC3/4,LHCF8,LHCF5,LHCF10) 和基因 (*PtPSY*, *PtPDS*, *PtVDE*) 被上调.
* 低光和HN的组合最大限度地提高了FX生产力,从第10天到9.82 mg/L/day.

结论:

* 的可用性显著调节了P. tricornutum*中的FX生物合成.
* 增强FCP形成和对特定胡卜素生成基因的上调是关键机制.
* 结合低光和高的战略种植提供了一种有效的方法来最大限度地提高FX产量.

关键词:

在FCP蛋白质中.菲奥达克提勒姆三角形.富可桑丁是一种富可桑丁.微藻是一种微藻.提供的可用性.

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