斯派克S2子单位:检测具有多个突变的新型SARS-CoV-2变体的可能目标
Teerada Ponpinit1, Yutthana Joyjinda1, Weenassarin Ampoot1
1Thai Red Cross Emerging Infectious Diseases Health Science Centre, King Chulalongkorn Memorial Hospital, Bangkok 10330, Thailand.
Tropical medicine and infectious disease
|February 23, 2024
概括
保存的SARS-CoV-2的S2子单元是分子诊断的一个有希望的目标,有效地检测新的变体,而不会影响RT-PCR测试性能.
科学领域:
- 病毒学 病毒学
- 分子诊断学 分子诊断
- 生物技术是生物技术.
背景情况:
- 新型SARS-CoV-2变种具有多种突变,可能会影响分子诊断分析的准确性.
- 斯派克 (S) 蛋白质的S2子单元在SARS-CoV-2变体中高度保存,这表明它作为稳定的诊断目标的实用性.
研究的目的:
- 评估SARS-CoV-2 S2子单元作为新型变异的诊断目标的有效性.
- 评估将S2原料和探头纳入现有的RT-PCR诊断套件的影响.
主要方法:
- 用商业RT-PCR套件对694个标本进行比较分析,与用S2原料和探头增强的相同套件对比.
- 对230,821个SARS-CoV-2核酸序列的生物信息分析,以评估S2原料和探针对循环变异的完整性.
- 对S2和其他基因 (E,N,ORF1ab) 的放大效率和Ct值的评估.
主要成果:
- 在任何测试样本中都没有观察到S2目标的放大问题,包括BA.4 Omicron变种.
- 在S2和其他基因 (E,N,ORF1ab) 或试剂批次之间,没有发现平均Ct值的统计学上显著差异.
- 生物信息分析表明,由于全球SARS-CoV-2变种中S2原料和探针的突变,性能问题的可能性非常低.
结论:
- SARS-CoV-2 S2子单元是分子诊断的可靠和保存的目标,能够检测出新的变异.
- 将S2检测集成到现有的RT-PCR测定中是可行的,而不会影响诊断灵敏度或特异性.
- 该S2子单位提出了一个有前途的战略,用于开发强大的诊断工具,用于当前和未来的SARS-CoV-2变种.
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