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外体中的微RNA通过白醇调解来激活神经元细胞

Zhendong Zhang^1,2, Qi Tao¹, Lixia Bai¹

¹Key Lab of New Animal Drug of Gansu Province, Key Lab of Veterinary Pharmaceutical Development of Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou 730050, China.

|February 23, 2024

概括

复星 (RSV) 刺激肠道细胞释放外体,从而激活神经元细胞. 这些外体含有特定的microRNAs促进神经细胞生长,这表明一种新的肠-大脑轴机制.

科学领域:

神经科学是一个神经科学.
细胞生物学细胞生物学
药理学药理学是指药理学的学科.

背景情况:

复星 (RSV) 在体外表现出与神经退行性疾病相关的神经保护性质.
肠-大脑轴和肠分泌物在神经元通信中的作用是积极研究的领域.
通过RSV影响肠-大脑相互作用的特定机制在很大程度上仍未被探索.

研究的目的:

调查复星 (RSV) 是否可以诱导肠-大脑相互作用.
为了确定RSV治疗的肠道细胞分泌的外体能否激活神经元细胞.
为了确定潜在的分子介质,如microRNAs,参与这种相互作用.

主要方法:

用RSV治疗Caco-2肠道细胞.
由Caco-2细胞分泌的外体被分离和表征 (传输电子显微镜,Illumina NovaSeq 6000).
评估了RSV处理的外体对SH-SY5Y神经细胞的影响,包括神经丝长度.
进行了miRNA测序,以分析外体体中的差异表达.

主要成果:

接受RSV治疗的Caco-2细胞分泌出激活SH-SY5Y神经细胞的外体,由增加的神经丝长度证明.
外体的表征证实了它们的存在和完整性.
与对照组相比,miRNA测序揭示了RSV处理的外体内26个miRNA的显著差异表达.
三种特定的miRNA被确定为诱导神经元激活的候选者,它们的模仿成功激活了SH-SY5Y神经元.

结论:

用RSV治疗的肠道细胞分泌出能够激活神经元细胞的外体.
这些外体内特定的微RNA在调解观察到的肠-大脑相互作用方面发挥着至关重要的作用.
这项研究突出了涉及RSV,肠道外体和miRNAs调节神经元活动的新奇机制.

关键词:

在Caco-2细胞中.在SH-SY5Y细胞中.外基因组是外基因组的组成部分.这是一个小RNARNA.复星醇是一种复星醇.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...