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遗传学分析,生物膜形成,抗微生物耐药性和这些特征之间的关系在Uropathogenic Escherichia coli中
Talieh Mostaghimi1,2, Abazar Pournajaf3, Ali Bijani4
1Student Research Committee, Babol University of Medical Sciences, Babol, Iran.
Molecular biology reports
|February 23, 2024
概括
这项研究发现,在尿路感染患者的尿病原菌大肠杆菌 (UPEC) 中,抗生素耐药性和扩展谱β-乳糖酶 (ESBL) 生产的高率. 基因组B2和D占主导地位,突显了持续监测的必要性.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 遗传学 是一个遗传学.
背景情况:
- 泌尿病原性大肠杆菌 (UPEC) 是尿路感染 (UTI) 的主要原因之一.
- 了解UPEC的特征,包括抗微生物耐药性和毒性因素,对于有效治疗至关重要.
- 特定的基因组,O血清组和粘附基因的流行可能会影响UPEC的致病性.
研究的目的:
- 为了调查儿科和成人尿路感染患者的UPEC分离物中遗传组,O血清组,粘附基因和抗菌素耐药性的流行情况.
- 评估与生物膜形成相关的基因表达水平和UPEC菌株中扩展光谱β-乳糖酶 (ESBL) 的存在.
- 确定UPEC中关键的遗传标记物和抵抗机制,从而导致尿路感染.
主要方法:
- 一项涉及156个尿路感染患者的UPEC分离物的横截面研究.
- 使用双盘协同 (DDS) 方法检测产生ESBL的分离物.
- 通过微板测定和基因表达分析评估生物膜形成,使用定量实时PCR.
- 使用PCR识别O血清组,粘附因子,ESBL和PMQR基因;通过多重PCR进行家族遗传分组.
主要成果:
- 在UPEC分离物中,耐药率很高,特别是对纳利迪克酸 (91.7%).
- 扩展光谱β-乳糖酶 (ESBL) 的产生在67.9%的隔离物中普遍存在.
- 基因组B2是最常见的 (37.1%),其次是D组. blaCTX-M和qnrS基因被广泛分布.
- 与生物膜生产商相比,非生物膜生产商的平均 fimA 基因表达显著更高 (p < 0.01).
结论:
- 高抗生素耐药性和ESBL产量强调需要进行局部耐药性监测和机制研究.
- 在UPEC分离物中,B2和D族群占主导地位,强调了它们的流行病学意义.
- 进一步调查流行病学方面以及在不同地区和时间范围内对UPEC分离物的特征是必不可少的.
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