Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

The impact of bedtime alignment on sleep health in older couples: gender-sensitive analysis.

BJPsych open·2026
Same author

Establishment of Dementia Platform Korea and the Trial Ready Registry to advance Alzheimer's disease research in Korea.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025
Same author

Therapeutic benefits of maintaining CDK4/6 inhibitors and incorporating CDK2 inhibitors beyond progression in breast cancer.

eLife·2025
Same author

Dementia Care Research and Psychosocial Factors.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025
Same author

Biomarkers.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025
Same author

Dual targeting of CDK4/6 and CDK7 augments tumor response and antitumor immunity in breast cancer models.

The Journal of clinical investigation·2025

相关实验视频

Updated: Jul 2, 2025

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
10:54

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations

Published on: September 17, 2012

10.5K

使用单细胞成像方法研究异质细胞周期进展.

Hee Won Yang1,2

  • 1Department of Pathology and Cell Biology, Columbia University Irving Medical Center, New York, NY, USA. hy2602@cumc.columbia.edu.

Methods in molecular biology (Clifton, N.J.)
|February 23, 2024
PubMed
概括

了解细胞循环进展是复杂的. 新的活单细胞成像和传感器揭示了细胞差异如何驱动多样化的反应,进步了细胞周期调节知识.

科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学
  • 生物物理学的生物物理.

背景情况:

  • 细胞循环进展研究受到复杂的调节网络和细胞变异性的阻碍.
  • 传统方法通常需要细胞同步,掩盖固有的生物差异.
  • 最近的技术进步提供了新的方法来研究细胞循环动力学,而不是同步.

研究的目的:

  • 为研究细胞循环进展提供一种新的活细胞成像工作流程.
  • 利用先进的活细胞传感器捕获细胞周期动态.
  • 研究细胞循环进展期间异质细胞反应背后的分子机制.

主要方法:

  • 开发一个全面的现场单细胞成像工作流程.
  • 利用尖端的活细胞传感器进行实时监测.
  • 在细胞循环动态中对细胞间异质性的分析.

主要成果:

  • 该工作流成功地在单细胞水平上捕捉了动态的细胞周期进展.
  • 活细胞传感器提供有关分子事件的高分辨率数据.
  • 细胞反应的异质性是观察到的和可量化的.

结论:

关键词:
细胞周期传感器 细胞周期传感器细胞对细胞的异质性异质性一个单细胞成像技术.

更多相关视频

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

14.3K
Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics
09:27

Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics

Published on: September 14, 2015

14.2K

相关实验视频

Last Updated: Jul 2, 2025

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
10:54

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations

Published on: September 17, 2012

10.5K
Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

14.3K
Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics
09:27

Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics

Published on: September 14, 2015

14.2K
  • 使用先进的传感器进行活细胞成像,克服了研究细胞循环进展的先前局限性.
  • 这种方法有助于阐明推动细胞周期异质性的分子机制.
  • 提出的工作流提供了一个强大的工具,用于未来的细胞循环研究.