Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

MicroRNAs01:22

MicroRNAs

3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Lower Eyelid Fat Pad Transfer and Upper Eyelid Blepharoplasty for Simultaneous Correction of Tear Trough and Sunken Upper Eyelid Deformities.

Aesthetic plastic surgery·2026
Same author

FOSL1-mediated super-enhancer facilitates pathological scarring.

Cell reports·2026
Same author

Adipose precursor cell-derived clusterin promotes homeostasis in subcutaneous adipose tissue.

Stem cell reports·2026
Same author

From Posttraumatic Lipoma to Hemangioma: 2 Foreign Bodies' 4-decade Unnoticed Presence.

Plastic and reconstructive surgery. Global open·2026
Same author

Mitochondrial dynamics in skin health and disease: energy, aging, and therapeutic perspectives.

Burns & trauma·2026
Same author

Effect of Nordic Walking on Anthropometrics, Glycemia, and Lipid Profile in Adults With Prediabetes or Diabetes: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.

Journal of diabetes research·2026

相关实验视频

Updated: Jul 2, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.6K

通过微阵列分析和体外实验来识别与体相关的微RNA.

Sichao Chen1, Wenchao Zhang1, Zenan Xia1

  • 1Department of Plastic and Reconstructive Surgery, Peking Union Medical college Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Molecular biotechnology
|February 23, 2024
PubMed
概括

五种微RNA (miRNA) 在 keloid 组织中被上调,在 keloid 病原发生过程中发挥关键作用. 这些miRNAs,包括hsa-miR-127-3p,代表了化体治疗的潜在治疗标.

关键词:
表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.凯洛伊德 (Keloid) 是一种类型的细胞.这是一个微型RNA.微阵列的微阵列在WGCNA中,WGCNA是WGCNA.

更多相关视频

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization
06:01

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization

Published on: June 7, 2016

6.8K
Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue
04:41

Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue

Published on: July 28, 2023

2.2K

相关实验视频

Last Updated: Jul 2, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.6K
Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization
06:01

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization

Published on: June 7, 2016

6.8K
Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue
04:41

Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue

Published on: July 28, 2023

2.2K

科学领域:

  • 分子生物学分子生物学
  • 遗传学 遗传学是一种遗传学.
  • 皮肤病学 皮肤病学

背景情况:

  • 微RNAs (miRNAs) 是基因表达的关键调节者.
  • 状体是由于细胞过程调节失调而产生的异常痕.
  • 鉴定参与 keloid 发育的特定 miRNA 对于治疗进步至关重要.

研究的目的:

  • 为了识别和验证关键的microRNAs (miRNAs) 与 keloid 病原发生相关.
  • 探索潜在的治疗性分子点,用于 keloid 治疗.
  • 为了阐明受确定 miRNAs 在 keloids 影响的调节途径.

主要方法:

  • 在GSE113620数据集上进行权重基因共表达网络分析 (WGCNA).
  • 微阵列分析和定量实时PCR (qRT-PCR) 用于miRNA表达的验证.
  • 生物信息分析包括基因本体学 (GO) 和KEGG通路丰富分析,用于目标基因预测.

主要成果:

  • 来自WGCNA的蓝色模块显示了与 keloids 的最高相关性.
  • 已经确定了五个枢纽miRNAs (hsa-miR-127-3p,hsa-miR-214-3p,hsa-miR-155-5p,hsa-miR-409-5p,hsa-miR-542-5p) 的存在.
  • 这五种miRNA在 keloid 组织中显著上调.
  • 目标基因在基因转录,蛋白质酸化,MAPK级联和PI3K-AKT信号通路中得到丰富.

结论:

  • 已识别的五种miRNAs (hsa-miR-127-3p,hsa-miR-155-5p,hsa-miR-214-3p,hsa-miR-409-5p,hsa-miR-542-5p) 在 keloid 病原发生过程中至关重要.
  • 这些miRNAs代表了 keloid 治疗的有前途的治疗点.
  • 这些发现突出了这些miRNAs在调节关键细胞途径中的作用,这些途径参与了 keloid 形成.