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相关概念视频

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
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2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

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Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
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2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

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Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
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相关实验视频

Updated: Jul 2, 2025

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM
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高灵敏度和高通量磁流CMOS细胞计与2D振荡器阵列和传感器间谱图交叉相关性

Hao Tang, Suresh Venkatesh, Zhongtian Lin

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    |February 23, 2024
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    这项研究引入了一种新的集成流量细胞计,使用磁传感器阵列进行高通量罕见细胞检测. 先进的信号处理显著提高了对于循环瘤细胞分析等应用的灵敏度和特异性.

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    科学领域:

    • 生物医学工程 生物医学工程
    • 微流体学 微流体学
    • 传感器技术 传感器技术

    背景情况:

    • 传统的流细胞计在高通量罕见细胞检测方面面临局限性,原因是灵敏度和特异性的权衡.
    • 通常需要水力动力聚焦,限制样本流量和增加复杂性.

    研究的目的:

    • 开发一台集成的流动细胞计,配备2D磁传感器阵列,用于高通量,敏感和特定的罕见细胞检测.
    • 为了克服高通量分析固有的灵敏度-特异性权衡.

    主要方法:

    • 一个集成的流量细胞计芯片,使用基于65纳米CMOS工艺的双频振荡器的2D磁传感器阵列.
    • 先进的信号处理,包括传感器光谱图的站点间交叉关联和两种不同的方法来抑制低频漂移.
    • 在7x7传感器阵列上使用磁标记的介电粒子和培养的淋巴瘤癌细胞进行演示.

    主要成果:

    • 在没有水力动力聚焦的情况下实现了高吞吐量,允许不受阻碍的样本流.
    • 通过跨站点交叉相关性,大幅抑制了错误检测概率,达到对百万分之零件 (sub-ppM) 水平的理论灵敏度.
    • 成功证明了该系统在检测磁标记颗粒和癌细胞方面的能力.

    结论:

    • 集成的流细胞计与2D磁传感器阵列提供了一个有希望的平台,敏感和特定的罕见细胞检测.
    • 开发的信号处理技术对于在保持精度的同时实现高吞吐量至关重要.
    • 这项技术在早期癌症诊断和监测方面具有很大的应用潜力,例如循环瘤细胞检测.