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记忆和调节细胞种群之间的平衡在移植接受者与操作耐受性的细胞种群

Caner Süsal^1,2, Cristiam M Alvarez³, Louise Benning⁴

¹Institute of Immunology, University of Heidelberg, Heidelberg, Germany.

Clinical and experimental immunology

|February 23, 2024

概括

在移植受体中监测T细胞子集可以预测移植的存活率. 调节细胞和减少的CD8+TEMRA T细胞的平衡表明操作耐受性,有助于识别患有移植损失风险的患者.

科学领域:

免疫学免疫学免疫学
移植生物学移植生物学
细胞免疫学细胞免疫学

背景情况:

捐赠者反应性记忆T细胞阻碍了长期移植的存活.
确定操作耐受性对于管理移植受体至关重要.
平衡全反应性记忆反应的调控机制是移植接受的关键.

研究的目的:

在移植受体中,研究记忆T细胞子集和调控性T或B细胞 (Tregs,Bregs) 之间的平衡.
确定免疫学标记,区分操作耐受性和慢性排斥.
分析与移植状况相关的Treg和Breg相关基因表达.

主要方法:

对移植接受者 (操作耐受性,慢性排斥,各种免疫抑制疗法),血液透析患者和健康对照者的前性研究.
流细胞计分析记忆T细胞子集和调节细胞频率.
对Treg和Breg相关分子的基因表达分析 (CD22,FoxP3,FcγRIIA/FcγRIIB比率).

主要成果:

与耐受性患者和对照人群相比,慢性排斥患者的CD8+终端分化功效记忆 (TEMRA) T细胞频率显著更高.
经营性耐受性患者表现出低CD8+TEMRAT细胞频率和Tregs和过渡Bregs的高频率.
耐受性患者表现出最低的CD8+ TEMRA T细胞与调节细胞的比率和明显的外周血液转录模式 (增加CD22,FoxP3;减少FcγRIIA/FcγRIIB比率).

结论:

CD8+ TEMRA T细胞和调节细胞 (Tregs,Bregs) 之间的平衡是移植耐受性的关键指标.
监测循环中的CD8+ TEMRA T细胞和调控细胞子集,以及它们的基因表达特征,可以帮助区分耐受性接受者和面临移植损失风险的人.
这种方法可能有助于个性化免疫抑制管理,并改善长期移植存活率.

关键词:

一切都是为了活跃.腎臟移植腎臟移植操作容忍度的使用.监管性B单元格的监管性B单元格

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