里斯林中的汽油注释 - 1,1,6-三甲基-1,2-二甲 (TDN) 选择性地激活人类气味受体OR8H1
Franziska Haag1, Tim Frey1,2, Lena Ball1,2
1Leibniz-Institute for Food Systems Biology at the Technical University of Munich, 85354 Freising, Germany.
Journal of agricultural and food chemistry
|February 23, 2024
概括
科学家们确定了一种特定的人类受体,OR8H1,用于在老年瑞斯林葡萄酒中发现的类似汽油的芳香化合物1,1,6-trimethyl-1,2-dihydronaphthalene (TDN). 这一发现解释了人类如何感知这种关键的葡萄酒香味.
科学领域:
- 葡萄酒学和葡萄种植
- 分子生物学分子生物学
- 感官科学 感官科学
背景情况:
- 葡萄藤 (Vitis vinifera) 是一种重要的水果作物,瑞斯林葡萄酒以其独特的老化汽油气味而闻名.
- 这种香气归因于1,1,6-三甲基-1,2-二甲 (TDN),其度因紫外线暴露和半球的影响而有所不同.
- TDN的感知是显著的,在澳大利亚的瑞斯林是可取的,但可能导致欧洲品种的拒绝,但人类受体仍然未被确定.
研究的目的:
- 确定负责检测1,1,6-三甲基-1,2-二纳 (TDN) 的特定人类气味受体.
- 为了了解在瑞斯林葡萄酒中TDN感知的分子基础.
主要方法:
- 使用基于细胞的HEK-293cAMP发光量测试对766种人类气味受体 (OR) 变异进行选.
- 使用GloSensor技术进行发光检测.
- 测试了受体反应与180个关键食物气味的图书馆,专注于TDN.
主要成果:
- 确定OR8H1是一种新型的气味受体,对TDN具有高度选择性.
- OR8H1对六碳环结构,包括TDN,进行了特殊调整.
- 这种受体的识别为TDN检测提供了分子基础.
结论:
- OR8H1是第一个被确定为TDN的人类受体,TDN是负责老年瑞斯林葡萄酒中类似汽油香气的关键化合物.
- 这一发现推动了我们对葡萄酒香味感知和对特定食物化合物嗅觉反应的遗传基础的理解.
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