试验和in silico方法用于牛乳糖蛋白-叶酸复合的阐明. 影响蛋白质结构和功能的分子变化
Leandro Fabián Bustos1, Franco Emanuel Vasile2, Oscar Edgardo Pérez3
1Universidad Nacional del Chaco Austral & CONICET - Instituto de investigaciones en procesos tecnológicos avanzados (INIPTA), Comandante Fernández 755, Presidencia Roque Sáenz Peña, 3700 Chaco, Argentina; Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica & CONICET-Universidad de Buenos Aires, Laboratorio Interdisciplinario de Dinámica Celular y Nano-Herramientas, Intendente Güiraldes 2160, CP 1428 Buenos Aires, Argentina.
Food research international (Ottawa, Ont.)
|February 23, 2024
概括
雄牛乳清蛋白缩物 (BWPC) 有效地将叶酸 (FA) 封装成纳米级复合物. 这种创新方法增强了抗氧化剂的活性,为食品和营养品行业提供了潜在的潜力.
科学领域:
- 食品科学 食品科学 食品科学
- 生物技术是生物技术.
- 营养保健品 营养保健品
背景情况:
- 雄牛乳清蛋白缩物 (BWPC) 是乳制品行业的一种副产品.
- 维生素是不稳定的生物活性化合物,需要进行保护性封装.
- 开发新的维生素纳米载体对于食品和营养药物应用至关重要.
研究的目的:
- 为了研究叶酸 (FA) 与BWPC的复合.
- 描述由此产生的BWPC-FA复合物的分子和功能性质.
- 探索BWPC作为维生素纳米载体的潜力.
主要方法:
- 分析技术:SEC-FPLC,光光谱学,FTIR,DLS,紫外线光谱学.
- 在体方法:分子对接.
- 在BWPC中确定了五种蛋白质类型.
主要成果:
- 叶酸 (FA) 在pH 7时通过H键和疏水相互作用与BWPC相互作用.
- 复杂化导致了BWPC蛋白质结构的构造变化.
- 在没有聚合的情况下,BWPC-FA复合体形成了纳米级结构 (100-150 nm).
- 通过分子对接,乳二对FA表现出最高的结合亲和力.
- 与单独BWPC相比,BWPC-FA复合物的抗氧化活性增加了20%.
结论:
- BWPC 作为叶酸 (FA) 的有效纳米载体.
- 复杂化过程增强了成分的抗氧化能力.
- 这项研究为提升水牛乳业副产品的价值提供了有价值的数据.
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