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用PacBio和Hi-C测序数据对云林牛的染色体水平基因组组合和注释

Zaichao Wei^1,2, Lilian Zhang^3,4,5, Lutao Gao^3,4,5

¹College of Food Science and Technology, Yunnan Agricultural University, Kunming, China.

Scientific data

|February 23, 2024

概括

研究人员开发了首个高质量的基因组组合,用于云林牛,一种新的牛肉品种. 这种遗传资源有助于了解牛的多样性,并推进云林牛的养殖计划.

科学领域:

动物基因组学动物基因组学
畜牧养殖畜牧养殖畜牧养殖
进行比较基因组学.

背景情况:

云灵牛是一种新型的牛肉品种,在中国云南开发,通过交叉繁殖布拉汉,穆雷灰和云南黄牛.
这种品种表现出适应热带/亚热带气候,良好的繁殖和增长率在热度/湿度下,抗寄生虫,以及理想的牛肉品质.
高质量的基因组组装对于理解这些特征的遗传基础以及未来的育种进步至关重要.

研究的目的:

为了产生优质的,染色体水平的基因组组合为云灵牛.
为云林牛研究和繁殖提供基础遗传资源.
为了进一步研究牛的遗传多样性和品种改进.

主要方法:

使用了短读数测序,PacBio HiFi测序和Hi-C支架技术的组合.
进行了染色体层次的基因组组装和定.
分析了基因组大小,重复序列和蛋白质编码基因.

主要成果:

成功生成了3.09Gb的染色体水平基因组组合,用于云灵牛.
该组件与31个染色体 (29个自体,X,Y) 连接,连接N50为35.97 Mb,支架N50为112.01 Mb.
确定了1.62 Gb的重复序列和20,660个蛋白质编码基因.

结论:

第一个云林牛基因组组合是一个重要的遗传资源.
该资源将促进对牛遗传多样性的研究.
它将加快选择性繁殖的努力,以改善云林牛.

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