Ursodeoxycholic 酸对过氧化引起的肝细胞损伤的改善作用和机制
Xueqin Wang1,2, Guangxi Liang2, Yang Zhou3
1Department of Thyroid Surgery, Affiliated Hospital of Southwest Medical University, Luzhou, 646000, Sichuan, China.
Scientific reports
|February 23, 2024
概括
乌尔索多氧胆酸 (UDCA) 保护肝细胞免受过氧化 (H2O2) 造成的损伤. 这项研究表明,UDCA可减少氧化应激和炎症,为肝损伤提供一种潜在的治疗方法.
科学领域:
- 肝病学 肝病学是一种肝病学.
- 氧化压力研究研究 氧化压力研究
- 药理学 药理学是指药理学的学科.
背景情况:
- 过氧化 (H2O2) 与肝细胞损伤和肝脏疾病有关.
- 氧化应激和炎症是H2O2诱导的肝损伤的关键机制.
- 在ApoE-/-小鼠中的超脂血症模型显示严重的肝损伤和高H2O2.2.
研究的目的:
- 评估ursodeoxycholic acid (UDCA) 对H2O2诱导的肝损伤的保护作用.
- 研究UDCA对氧化应激标志物和肝细胞炎症基因表达的影响.
- 建立UDCA在肝损伤治疗中的治疗应用的理论基础.
主要方法:
- 在体外研究中,使用暴露于H2O2的L02细胞来模拟氧化应激.
- 在体内实验中,使用高脂肪饮食的ApoE-/-小鼠诱导高脂血和肝损伤.
- 分析反应性氧物种 (ROS),马隆迪化物 (MDA),超氧化物失调酶 (SOD) 和相关基因转录.
主要成果:
- 在受H2O2损伤的L02细胞中,UDCA显著改善了氧化应激 (P < 0.05).
- UDCA治疗导致炎症和氧化应激相关基因的转录水平显著改善 (P <0.05).
- 在小鼠模型中,H&E染色揭示了严重的肝损伤,检测出过多的血清H2O2.
结论:
- 乌尔索西胆酸显示出显著的抗炎和抗氧化应激效应.
- 在L02肝细胞中,UDCA提供了对H2O2诱导的损伤的保护作用.
- 这些发现支持UDCA作为治疗性肝损伤与氧化应激相关的治疗剂的潜力.
相关概念视频
01:24Peroxisomes
12.4K
Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
12.4K
02:04Oxidation of Alkenes: Anti Dihydroxylation with Peroxy Acids
5.8K
Diols are compounds with two hydroxyl groups. In addition to syn dihydroxylation, diols can also be synthesized through the process of anti dihydroxylation. The process involves treating an alkene with a peroxycarboxylic acid to form an epoxide. Epoxides are highly strained three-membered rings with oxygen and two carbons occupying the corners of an equilateral triangle. This step is followed by ring-opening of the epoxide in the presence of an aqueous acid to give a trans diol.
5.8K
02:44Oxidation of Alkenes: Syn Dihydroxylation with Osmium Tetraoxide
10.2K
Alkenes are converted to 1,2-diols or glycols through a process called dihydroxylation. It involves the addition of two hydroxyl groups across the double bond with two different stereochemical approaches, namely anti and syn. Dihydroxylation using osmium tetroxide progresses with syn stereochemistry.
10.2K
02:17Reduction of Alkenes: Asymmetric Catalytic Hydrogenation
3.3K
Catalytic hydrogenation of alkenes is a transition-metal catalyzed reduction of the double bond using molecular hydrogen to give alkanes. The mode of hydrogen addition follows syn stereochemistry.
The metal catalyst used can be either heterogeneous or homogeneous. When hydrogenation of an alkene generates a chiral center, a pair of enantiomeric products is expected to form. However, an enantiomeric excess of one of the products can be facilitated using an enantioselective reaction or an...
The metal catalyst used can be either heterogeneous or homogeneous. When hydrogenation of an alkene generates a chiral center, a pair of enantiomeric products is expected to form. However, an enantiomeric excess of one of the products can be facilitated using an enantioselective reaction or an...
3.3K
01:20Radical Autoxidation
2.1K
The oxidation of an organic compound in the presence of air or oxygen is called autoxidation. For example, cumene reacts with oxygen to form hydroperoxide. Autoxidation involves initiation, propagation, and termination steps. Many organic compounds are susceptible to autoxidation—especially ethers in the presence of oxygen, which form hydroperoxides. Even though this reaction is slow, old ether bottles contain small amounts of peroxide, which leads to laboratory explosions during ether...
2.1K
02:37Oxidation of Alcohols
13.1K
In this lesson, the oxidation of alcohols is discussed in depth. The various reagents used for oxidation of primary and secondary alcohols are detailed, and their mechanism of action is provided.
The process of oxidation in a chemical reaction is observed in any of the three forms:
The process of oxidation in a chemical reaction is observed in any of the three forms:
13.1K