斯芬戈蒙纳斯 (Sphingomonas flavescens sp.) 是一种有毒的植物. 从土壤中分离出来的nov
Hyosun Lee1, Dhiraj Kumar Chaudhary2, Dong-Uk Kim3
1Department of Biological Science, College of Science and Engineering, Sangji University, Wonju, 26339, Republic of Korea.
Archives of microbiology
|February 24, 2024
概括
一种新型的有氧细菌,Sphingomonas flavescens菌株PT-12T,从农业土壤中分离出来. 多相分类学分析证实,它代表了Sphingomonas属内的新物种.
科学领域:
- 微生物学 微生物学
- 细菌学 细菌学是一门学科.
- 分类学 分类学是指分类学.
背景情况:
- 土壤拥有各种微生物群落,对生态系统健康至关重要.
- 准确的细菌分类对于理解微生物的作用和相互作用至关重要.
- 斯芬哥马纳斯属以其代谢的多功能性和在各种环境中的存在而闻名.
研究的目的:
- 从农业土壤中分离和描述一种新的有氧细菌.
- 用多相方法确定孤立菌株的分类学位置.
- 建议在Sphingomonas属中提出一种新物种.
主要方法:
- 从农业土壤中分离和培养有氧细菌.
- 格拉姆染色,运动性测试和生物化学测试 (催化酶,氧化酶).
- 对生长条件 (温度,盐度,pH值) 的生理测试.
- 16S rRNA基因测序和遗传学分析.
- 基因组测序以确定基因组大小和DNA G+C含量.
- 平均核酸标识 (ANI) 和数字DNA-DNA杂交 (dDDH) 的计算.
- 化学分类学分析,包括脂肪酸分析和极性脂质分析.
主要成果:
- 菌株PT-12T是一种格拉姆阴性,非运动性,棒状的有氧细菌.
- 在18-37°C,0-1.0%NaCl和pH值6.0-8.0.0之间观察到最佳生长.
- 遗传学分析将PT-12T菌株置于Sphingomonas属内,16S rRNA与S. limnosediminicola (98.4%) 的相似性最高.
- 基因组大小为 2,656,862 bp,DNA G+C 含量为 63.5%.
- ANI和dDDH值表明与相关物种的区别,最高值为S. segetis YJ09T (76.2%的ANI,20.2%的dDDH).
- 化学分类学数据 (Q-10,双,特定脂肪酸和极性脂质) 支持了它的分类.
结论:
- 多相数据强烈支持将PT-12T菌株归类为新物种.
- 这种新物种的拟议名称是Sphingomonas flavescens sp. 在新的一年里.
- 类型菌株是PT-12T (KCTC 92114T=NBRC 115717T) 的.
关键词:
人类的家谱 (Phylogeny) 是一个学科.土壤 土壤土壤.斯芬戈蒙纳达科 (Sphingomonadaceae) 是一个植物.斯芬戈蒙纳斯 (Sphingomonas flavescens sp.) 是一种有毒的植物. 在新的一年里.分类学 分类学是指分类学.更多相关视频
相关概念视频
Bacterial Phylum Planctomycetes
542
Planctomycetes are a group of morphologically distinct bacteria predominantly classified into two orders: Planctomycetales and Brocadiales. These gram-negative bacteria exhibit unique features, including division by budding and the presence of stalks or appendages. Their cells are often found in rosette arrangements, and they are notable for possessing an S-layer in their cell envelope, which is relatively uncommon among bacteria. Additionally, Planctomycetes frequently exhibit intracellular...
542
Bacterial Phylum Spirochaetes
1.3K
Spirochetes, unique bacteria in the phylum Spirochaetes, are gram-negative, motile, tightly coiled, slender, and flexible. They inhabit aquatic sediments and animals, with some causing diseases like syphilis. Spirochetes are classified into eight genera based on habitat, pathogenicity, phylogeny, and characteristics.Their distinctive motility arises from endoflagella, located within the cell’s periplasm. These endoflagella anchor at the cell poles and extend along the cell length, encased...
1.3K
Bacterial Phylum Bacteroidota
888
The phylum Bacteroidota includes over 700 species classified into four primary orders: Bacteroidales, Cytophagales, Flavobacteriales, and Sphingobacteriales. These gram-negative, non-sporulating rods exhibit saccharolytic capabilities and can be aerobic or fermentative, encompassing obligate aerobes, facultative aerobes, and obligate anaerobes. Many species display gliding motility, though some are nonmotile or use flagella. The genus Bacteroides is well-studied due to its significant role in...
888
Anoxygenic Phototrophic Bacteria
1.2K
Anoxygenic phototrophic bacteria are a diverse group of microorganisms that perform photosynthesis without producing oxygen. They primarily include purple sulfur bacteria, purple nonsulfur bacteria, green sulfur bacteria, and green nonsulfur bacteria. These bacteria are classified into the Gammaproteobacteria, Alphaproteobacteria, Betaproteobacteria, Chlorobi, and Chloroflexi lineages, each with distinct physiological and ecological adaptations.Purple sulfur bacteria belong to the...
1.2K
Bacterial Phylum Tenericutes
599
The phylum Tenericutes, which includes the single class Mollicutes, comprises bacteria that lack cell walls. The term "Mollicutes" derives from the Latin word mollis, meaning "soft." These organisms are among the smallest known and are commonly referred to as mycoplasmas due to the prominence of the genus Mycoplasma, which includes well-known human pathogens. Despite their inability to stain gram-positively (a result of their lack of cell walls), mycoplasmas are phylogenetically related to the...
599
Microbe-Plant Interactions
85
Microbe-plant interactions represent a dynamic spectrum of associations shaped by intricate chemical signaling. These interactions can be neutral, beneficial, or detrimental, and profoundly influence plant physiology, growth, and ecosystem function. The plant microbiome, comprising bacteria, fungi, archaea, protists, and viruses, plays a pivotal role in mediating these effects through surface colonization, internal colonization, or systemic symbiosis.Mutualistic associations, particularly with...
85


