在Set5p介导的酸应激反应中的差异性蛋白质表达和工程酵母应激耐受性的新目标
Ming-Ming Zhang1, Bing Yuan1, Ya-Ting Wang1
1State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Journal of proteome research
|February 24, 2024
概括
发芽酵母Saccharomyces cerevisiae使用组蛋白修饰来耐受酸压力,这是林氏纤维素生物转化中的一个关键挑战. Set5p和染色体调节器对于这种应激反应至关重要.
科学领域:
- * 分子生物学 * 分子生物学
- * 表观遗传学 是一种表观遗传学.
- * 酵母遗传学公司
背景情况:
- * 酸在纤维素化物中抑制了微生物的生长和生物生产.
- * 质子修饰和染色体重塑对于真核细胞代谢至关重要,但它们在酸应激中的作用尚不清楚.
- *之前的研究表明,Set5p的过度表达增强了Saccharomyces cerevisiae中的酸耐受性.
研究的目的:
- * 调查酸应激反应中素H4甲基转移酶Set5p的作用.
- * 分析在乙酸压力下全球蛋白质表达变化.
- * 确定参与酵母应激耐受性的关键调节者.
主要方法:
- * 全球蛋白质表达分析.
- *对特定蛋白质 (Rtc3p,Ies3p,Taf14p) 的功能研究.
- * 在*Saccharomyces cerevisiae*中进行压力反应测定.
主要成果:
- * 显著激活细胞内蛋白质表达,特别是染色质修饰,信号转导和碳水化合物代谢.
- * 在乙酸压力下增加Set5p表达.
- * 限制端粒封闭蛋白Rtc3p,Ies3p和Taf14p对于应激反应至关重要.
结论:
- *Set5p在酵母酸乙酸应激耐受性中起着重要作用.
- * 涉及基因组修饰和色素调节的表观遗传机制是酵母应激反应的关键.
- *这些发现有助于开发强大的酵母菌株,用于细胞菌生物转化.
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