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在摩洛哥的小反动物虫病毒的血清和分子表征

Barbara Colitti¹, Soukaina Daif², Imane Choukri²

¹Department of Veterinary Science, University of Turin, Largo Braccini 2, 10095 Grugliasco, TO, Italy.

Animals : an open access journal from MDPI

|February 24, 2024

概括

摩洛哥的绵羊和山羊林氏病毒 (SRLV) 循环揭示了新的B和重组A/B亚型. 监测病毒演变对于开发准确的诊断测试至关重要,尤其是在实施控制措施的情况下.

科学领域:

兽医病毒学兽医病毒学
分子流行病学分子流行病学
动物健康动物健康

背景情况:

绵羊和山羊的晶状病毒 (SRLV) 是影响全球家禽反动物的重要病原体.
了解SRLV菌株的起源和分布对于有效的疾病管理至关重要.
之前的研究已经为SRLV流行病学提供了洞察力,但摩洛哥的数据仍然有限.

研究的目的:

调查SRLV在摩洛哥的传播和遗传多样性.
评估SRLV在摩洛哥国内反动物中的流行情况.
为了识别新的SRLV亚型和重组菌株.

主要方法:

总共有51个农场在摩洛哥不同地理位置被选中.
查和基因定型ELISA用于血清学测试.
从全血中提取DNA,然后进行嵌套的口PCR.
桑格和下一代测序 (NGS) 的安普利康测序被用于基因分析.

主要成果:

该研究估计SRLV患病率低于20% (95%CI).
显著比例的查阳性样本显示在血清型的差异.
通过序列分析确定了新的SRLV B和重组A/B亚型.
检测到一种SRLV A亚型,在血清型化表位上有不匹配.

结论:

摩洛哥的SRLV流通的特点是存在新型和重组菌株.
现有的诊断工具,特别是基因型定型ELISA,可能难以检测不同的菌株.
持续监测病毒序列和进化是开发特定和有效的诊断测试的必要条件.
管理实践可能会影响SRLV的内在传播.

关键词:

安普利康序列的测序方法不同的不同菌株.血清类型的确定.小反动物的lentiviruses病毒.

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