用SNP基因定型数据分析罗马尼亚卡尔帕蒂纳山羊的遗传多样性
Bogdan Alin Vlaic1, Augustin Vlaic2, Isa-Rita Russo3
1Department of Animal Breeding, Faculty of Animal Science and Biotechnologies, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Mănăștur Street 3-5, 400372 Cluj-Napoca, Romania.
Animals : an open access journal from MDPI
|February 24, 2024
概括
这项研究描述了罗马尼亚的卡尔帕蒂纳山羊遗传资源,尽管有效种群规模下降,但内生繁殖率很低. 这些发现有助于这一重要的牲畜品种的遗传改进.
科学领域:
- 动物遗传学动物遗传学
- 基因组资源 基因组资源
- 畜牧养殖 畜牧养殖 畜牧养殖
背景情况:
- 畜牧业,包括山羊养殖,有着古老的根源,在1980年后,罗马尼亚出现了系统的品种改进.
- 罗马尼亚缺乏对其山羊种群的全面基因组表征,尽管它们在经济上越来越重要.
- 以前对罗马尼亚山羊的遗传研究是有限的,使用微卫星和mtDNA.
研究的目的:
- 分析罗马尼亚四个地理区域的卡尔帕蒂纳山羊的遗传变异性.
- 评估与海拔高度和温度相关的异构性,近亲繁殖系数和有效种群规模.
- 为Carpatina山羊品种的遗传改进提供基础数据.
主要方法:
- 使用Illumina OvineSNP60高密度芯片对来自不同地理区域的67只卡帕蒂纳山羊进行基因组DNA分析.
- 计算异构性,近亲繁殖系数和跨自体的有效种群大小 (N).
- 单核酸多态 (SNP) 数据质量控制和链接不平衡 (LD) 的分析.
主要成果:
- 预期的异性 (H) 范围从0.413 (低温) 到0.420 (高温) 的环境.
- 在高温组中观察到较低的正平均内生系数 (0.009);整体内生影响较低.
- 实际人口规模 (N) 最近出现了下降,值为123 (高海拔),125 (低海拔),185 (高温),92 (低温).
结论:
- 卡尔帕蒂纳山羊种群表现出较低的近亲繁殖水平.
- 有效人口大小 (N) 趋势表明,在上个世纪有显著的下降.
- 这种基因组表征为卡尔帕蒂纳品种的有针对性的繁殖计划和保护策略提供了关键的见解.
相关概念视频
Comparing Copy Number Variations and SNPs
17.7K
Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
17.7K
Single Nucleotide Polymorphisms-SNPs
15.1K
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
15.1K
Genome-wide Association Studies-GWAS
13.4K
Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...
GWAS does not require the identification of the target gene involved in...
13.4K
Incomplete Dominance
22.6K
Gregor Mendel's work (1822 - 1884) was primarily focused on pea plants. Through his initial experiments, he determined that every gene in a diploid cell has two variants called alleles inherited from each parent. He suggested that amongst these two alleles, one allele is dominant in character and the other recessive. The combination of alleles determines the phenotype of a gene in an organism.
22.6K


