橄厂废水提取物富含氧和醇调节IPEC-J2细胞中的宿主-病原体相互作用
Flavia Ferlisi1, Chiara Grazia De Ciucis2, Massimo Trabalza-Marinucci1
1Department of Veterinary Medicine, University of Perugia, 01623 Perugia, Italy.
Animals : an open access journal from MDPI
|February 24, 2024
概括
橄厂废水 (OMWW) 聚醇对猪细胞中的沙门氏菌具有抗微生物和免疫调节作用. 这表明OMWW提取物可能是农场动物有益的料补充剂,降低疾病风险.
科学领域:
- 动物科学动物科学
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
背景情况:
- 橄油副产品,如橄厂废水 (OMWW),为动物料补充剂提供了可持续的来源.
- 肠道疾病和牲畜对抗生素的耐药性,特别是来自诸如沙门氏菌 (Salmonella Typhimurium) 这样的病原体,在畜牧业中构成了重大挑战.
研究的目的:
- 为了研究OMWW提取物多 (OMWW-EP) 对感染Salmonella Typhimurium的猪肠上皮细胞系 (IPEC-J2) 的免疫调节和抗菌作用.
- 评估OMWW-EP对IPEC-J2细胞中的细菌侵入性,基因表达和细胞因子释放的影响.
主要方法:
- IPEC-J2细胞先用OMWW-EP (0.35和1.4μg) 进行预处理,然后感染沙门氏菌.
- 量化了细菌的侵入性,并使用RT-qPCR分析了基因表达 (IL1B,MYD88,DEFB1,DEFB4A,TGFB1).
- 细胞因子释放通过ELISA测量.
主要成果:
- 1.4微克的OMWW-EP显著降低了沙门氏菌Typhimurium的入侵性.
- 较低度 (0.35μg) 调节了与免疫相关的基因 (IL1B,MYD88,DEFB1,DEFB4A) 的表达.
- 较高度 (1.4μg) 改变了IL1B,DEFB4A和TGFB1的表达,而总体细胞因子含量没有显著变化.
结论:
- 富含多的OMWW提取物在猪肠细胞中表现出体外免疫调节和抗微生物特性,对抗猪肠细胞中的沙门氏菌Typhimurium.
- 这些发现支持OMWW多作为天然料补充剂的潜力,以增强肠道健康并减少猪的病原体负载.
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