两种Streptococcus suis血清型8菌株的比较基因组分析确定了两种新的病毒性相关基因
Qi Sheng1,2,3, Qiuhua Xu1,2,3, Zouran Lan1,4
1MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210014, China.
Animals : an open access journal from MDPI
|February 24, 2024
概括
测序了Streptococcus suis血清型8的完整基因组,揭示了毒性菌株中独特的基因. 这些基因,包括MarR和ATPase,对于S. suis的毒性和潜在的水平传播至关重要.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 病原体研究 病原体研究
背景情况:
- 瑞杆菌 (Streptococcus suis) 是一种重要的动物传染病原体,在猪和人类中引起脑膜炎和败血症.
- 血清型8种S. suis菌株的检测率很高,并感染多个宿主,但它们的完整基因组仍然未被鉴定.
研究的目的:
- 测序和分析毒性和非毒性S. suis血清型8菌株的完整基因组.
- 在病毒性S. suis血清型8中识别独特的遗传元素和可疑的毒性相关基因 (PVGs).
- 调查已识别的PVG在S. suis病原体中的作用.
主要方法:
- 病毒性 (2018WUSS151) 和非病毒性 (WUSS030) S. suis 血清型 8 株的全基因组测序.
- 对比基因组分析以识别独特的基因和基因组差异.
- 在小鼠感染模型中,构建和测试删除突变的假定毒性相关基因 (PVG) 的毒性.
主要成果:
- 毒性和非毒性S. suis血清型8菌株之间的高基因组同样性 (99.68%),其中prophages是关键差异.
- 在毒性菌株中识别了83个独特基因,包括三个PVG (padR,marR,atpase).
- 两个PVG,padR和marR,位于能够形成圆形DNA的转子中,表明水平基因转移.
- 在小鼠模型中,marR或atpase的删除突变显示了减弱的毒性,证实了它们在S. suis病原发生中的作用.
结论:
- 这项研究为S. suis血清型8菌株提供了第一个完整的基因组序列.
- 独特的prophages和独特的基因,特别是像marR和atpase这样的PVGs,有助于S. suis血清型8的毒性.
- 这些发现增强了我们对S. suis血清型8的基因组特征和发病机制的理解,突出了潜在的毒性和传播机制.
更多相关视频
相关概念视频
Genome Size and the Evolution of New Genes
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
Bacterial Translocation and Protein Secretion
Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
Modern Molecular Taxonomy
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Evolution of Microbial Genome
Microbial genome evolution is a highly dynamic process shaped by continual gene gain and loss across species and strains. This genomic flexibility allows microorganisms to adapt rapidly to environmental pressures and interactions with other organisms. Central to understanding this diversity is the distinction between the core and pan genomes.The core genome comprises the genes shared by all sampled strains of a species, representing essential functions needed for fundamental cellular processes.
Determinants of Bacterial Pathogenicity and Virulence
Pathogenic bacteria employ a variety of strategies to establish infections, including the secretion of extracellular enzymes that act as potent virulence factors. These enzymes facilitate bacterial colonization of host tissues and help evade immune surveillance. By targeting structural components of host tissues and interfering with immune mechanisms, these enzymes play a pivotal role in disease progression.Extracellular Enzymes Facilitating Tissue Invasion: Several bacterial pathogens secrete...
Regulation of Bacterial Virulence
Pathogenic bacteria employ a range of regulatory mechanisms to modulate the expression of virulence genes in response to environmental and host-derived signals. These mechanisms ensure that virulence factors are expressed only under favorable conditions, thereby optimizing infection and survival strategies.Mechanisms of Virulence RegulationKey regulatory strategies include:Two-Component Systems: These consist of a membrane-bound sensor kinase and a cytoplasmic response regulator. Environmental...


