转录组数据揭示了shitou的肌细胞繁殖和分化过程中的circRNA-miRNA-mRNA调节网络
Rongqin Huang1,2, Jiahui Chen1,2, Xu Dong1,2
1College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Animals : an open access journal from MDPI
|February 24, 2024
概括
循环RNAs (circRNAs) 通过海绵式微RNAs (miRNAs) 调节的肌肉生长. 这项研究揭示了关键的circRNA-miRNA-mRNA网络和基因参与了Shitou的肌体发生,为肌肉发育调节提供了洞察力.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 动物科学动物科学
背景情况:
- 循环RNAs (circRNAs) 是基因表达的新兴调节者,作为miRNA海绵.
- 肉是一种有价值的商品,但肌肉发育的分子机制尚未得到充分探索.
- 了解circRNA的作用对于提高的肌肉生长和质量至关重要.
研究的目的:
- 为了研究的肌细胞和肌管中circRNAs和mRNAs的表达特征.
- 为了确定circRNA-miRNA-mRNA相互作用控制肌肉发育在希图.
- 为了阐明的肌肉形成中circRNAs的调节网络.
主要方法:
- 在的肌细胞和肌管上进行了RNA测序,以描述circRNA和mRNA的表达.
- 生物信息分析确定了差异表达的circRNAs (DEcircRNAs) 和mRNAs (DEmRNAs).
- 计算工具 (RNAhybrid,miRDB,Cytoscape) 用于构建circRNA-miRNA-mRNA网络并识别枢纽基因.
主要成果:
- 确定了96个DEcircRNA和880个DEmRNA,其中父母基因在Wnt信号通路中得到了丰富.
- 构建了一个全面的circRNA-miRNA-mRNA相互作用网络,涉及93个circRNA,351个miRNA和305个mRNA.
- 确定了10个可能与肌体发生有关的枢纽基因,以及它们的调控性circRNA-miRNA-枢纽基因轴.
结论:
- 循环RNA在通过ceRNA网络调节肌肉生长和分化方面发挥着重要作用.
- Wnt信号通路与的肌细胞增殖和分化有关.
- 这项研究为的肌肉发育的分子机制提供了新的见解,由circRNA-miRNA-mRNA相互作用驱动.
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