开发基于化曲线的三重EVA绿色实时PCR试验,用于同时检测三种病原体
Xuan Dong1,2, Yujin Chen1,3, Haoyu Lou1
1State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Laboratory for Marine Fisheries Science and Food Production Processes, Laoshan Laboratory, Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Qingdao 266071, China.
Animals : an open access journal from MDPI
|February 24, 2024
概括
一种新的Eva Green实时PCR测定方法可以准确地同时检测出三种主要的病原体:肠细胞动物肝炎 (EHP),传染性皮下和血液形成性缩病毒 (IHHNV) 和Decapod虹色病毒1 (DIV1). 这种方法为病管理提供了可靠的工具.
科学领域:
- 水产养殖是水产养殖的一种方式.
- 兽医病毒学 兽医病毒学
- 分子诊断学 分子诊断
背景情况:
- 养殖面临着严重的经济损失,原因是感染了Enterocytozoon hepatopenaei (EHP),感染性皮下和造血性亡病毒 (IHHNV) 和Decapod虹彩病毒1 (DIV1).
- 准确且同时检测这些病原体对于有效的疾病管理和预防在产业至关重要.
研究的目的:
- 开发和验证一种新的,基于化曲线的三重实时PCR试验,用于同时检测EHP,IHHNV和DIV1.
- 评估开发的试验的特异性,灵敏性和可重复性.
主要方法:
- 建立使用Eva Green光染料的三重实时PCR测定方法,同时检测EHP,IHHNV和DIV1.
- 使用来自中国各省的临床样本验证该试验.
- 结果与已建立的TaqMan定量PCR (qPCR) 试验进行比较.
主要成果:
- 三重Eva Green实时PCR测定显示出高特异性,灵敏性和可重复性.
- 对190个临床样本的分析显示,EHP的阳性率为10.5%,IHHNV为18.9%,DIV为44.2%.
- 开发的测定方法与TaqMan qPCR测定方法对所有三种病原体的百分之百一致.
结论:
- 新开发的三重Eva Green实时PCR测定是一种可靠和准确的方法,用于同时检测EHP,IHHNV和DIV1.
- 这种测试为在水产养殖环境中快速有效诊断关键的病提供了宝贵的工具.
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