猪内源性逆转录病毒 (PERV) 在猪基因组中的逆转录病毒插入多态性 (RIP)
Zhanyu Du1,2, Cai Chen1, Yao Zheng1
1College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Animals : an open access journal from MDPI
|February 24, 2024
概括
移动全长内源逆转录病毒 (Fl-ERVs) 在猪中产生遗传变异. 这项研究确定并证实了与表型变化相关的逆转录病毒插入多态性 (RIP),如体重和基因表达,突出了Fl-ERVs.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 动物遗传学动物遗传学
背景情况:
- 内源逆转录病毒 (ERV) 是LTR逆转录子,占哺乳动物基因组的很大一部分.
- 虽然许多ERV是不活跃的,但一些全长ERV (Fl-ERV) 在猪基因组中保持了移动性.
- 由Fl-ERVs产生的逆转录病毒插入多态 (RIP) 是猪遗传和表型变异的潜在来源.
研究的目的:
- 为了研究在猪中Fl-ERV调动产生的未开发的RIP.
- 评估Fl-ERV衍生的RIPs对表型变异的影响.
- 了解Fl-ERVs在促进猪遗传多样性的生物学作用.
主要方法:
- 进行比较基因组分析来预测RIP.
- 聚合酶链反应 (PCR) 和测序用于RIP确认.
- 种群和关联分析以评估RIP多态和表型关联.
主要成果:
- 预计有39个复审期,其中18个通过PCR证实.
- 四个RIP地点 (D5,D14,D15,D18) 显示了多种猪品种的多态性.
- 插入 STAB2 样基因的 ERV-D14 RIP 与体重变化有关,并在不同组织中改变了 STAB2 样基因表达.
结论:
- 在猪基因组中,fl-ERVs正在积极动员,产生RIP.
- 这些RIP有助于基因组变异,并影响表型特征,包括生长和基因表达.
- 在猪种群的遗传多样性和适应中,fl-ERVs起着重要作用.
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