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SARS-CoV-2 感染改变了脂肪细胞的表型和基因表达

Paola Quaranta^1,2, Gaia Scabia^3,4, Barbara Storti⁵

¹Retrovirus Center, Virology Section, Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Via Savi 10, 56126 Pisa, Italy.

International journal of molecular sciences

|February 24, 2024

概括

过多的脂肪质量增加了严重的COVID-19风险. 这项研究表明,SARS-CoV-2感染了人类脂肪细胞,导致炎症和脂肪细胞功能改变,影响COVID-19的进展.

科学领域:

细胞生物学细胞生物学
病毒学病毒学
代谢性疾病代谢性疾病

背景情况:

过度肥胖与严重的COVID-19结局有关.
目前尚不清楚SARS-CoV-2与脂肪细胞之间的相互作用.
了解COVID-19在脂肪组织中的进展对于长期健康至关重要.

研究的目的:

为了研究人类脂肪细胞中的SARS-CoV-2感染性.
确定SARS-CoV-2感染对脂肪细胞功能和形态学的影响.
为了探索脂肪细胞在COVID-19病变发生过程中的作用.

主要方法:

使用一个体外模型与人类SGBS (辛普森-戈拉比-贝梅尔综合征) 脂肪细胞.
通过测量病毒基因组释放,核囊体检测和尖端蛋白免疫染来评估SARS-CoV-2感染.
分析了与炎症,氧化应激和感染后脂肪细胞功能相关的基因表达的变化.

主要成果:

表达ACE2的SGBS脂肪细胞易受SARS-CoV-2感染.
受感染的脂肪细胞表现出增加的脂质液滴大小,高调的炎症基因表达 (Haptoglobin,MCP-1,IL-6,PAI-1),以及升高的氧化应激 (MnSOD).
SARS-CoV-2 感染减少了关键脂肪细胞功能的转录,包括勒和脂肪酸合成酶.

结论:

人类SGBS脂肪细胞可以被SARS-CoV-2感染.
感染SARS-CoV-2会导致脂肪细胞的显著形态和功能变化.
这些发现凸显了脂肪细胞作为潜在的目标和对COVID-19严重程度和长期并发症的贡献者.

关键词:

在 COVID-19 疫情中,在SGBS中,SGBS是SGBS.这是一种炎症炎症炎症炎症.脂肪液滴滴滴滴滴滴滴滴滴滴滴滴滴滴滴

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