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会损害皮肤纤维细胞和小鼠的伤口愈合过程

Sara R Dresler¹, Bronson I Pinto¹, Matthew C Salanga¹

¹Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011, USA.

International journal of molecular sciences

|February 24, 2024

概括

无机暴露会损害雌性小鼠的伤口愈合,影响皮肤细胞并减缓关闭. 这种性别特异效应突显了对污染的环境健康问题.

科学领域:

环境健康环境健康
毒理学毒理学毒理学
皮肤病学皮肤病学

背景情况:

无机 (NaAsO2) 是一个全球水污染物,超过了EPA限制.
暴露与各种健康问题有关,但其对伤口愈合的影响尚不清楚.

研究的目的:

调查环境相关的度对伤口愈合的影响.
为了确定是否以性别特定的方式影响伤口愈合.

主要方法:

在体外:皮肤纤维细胞暴露于NaAsO2以评估迁移,增殖和生存能力.
在体内:C57BL/6小鼠暴露于NaAsO2,随后进行全厚背部伤口的创建和评估.
皮肤组织的基因表达分析.

主要成果:

在体外:NaAsO2降低了纤维细胞迁移,增殖和活力 (LOEL 10μM).
在1μM NaAsO2.2.下观察到的MMP1转录丰富.
在体内:雌性小鼠的伤口闭合减少,红斑增加;雄性小鼠没有.
雌性小鼠在伤口皮肤中表现出As3mt和Esr2mRNA的增加.

结论:

环境相关的含量会对伤口愈合产生负面影响.
对伤口愈合的有害影响取决于性别,主要在女性身上观察到.
需要进一步的研究,以阐明在伤口修复中特定于性别的毒性背后的机制.

关键词:

作为一个3mt.在MMP1中,MMP1是MMP1.内分泌干扰干扰内分泌系统环境污染物环境污染物划痕测试试验划痕测试试验

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