整体转录组分析和功能预测长非编码RNA与日本米品种的寒冷耐受性相关
1Key Laboratory of Germplasm Enhancement and Physiology & Ecology of Food Crop in Cold Region, Ministry of Education/College of Agriculture, Northeast Agricultural University, Harbin 150030, China.
International journal of molecular sciences
|February 24, 2024
概括
低温压力影响大米产量. 这项研究揭示了一种新的竞争性内源性RNA (ceRNA) 网络,涉及长非编码RNA,microRNA和信使RNA,调节大米中的抗氧化反应.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 低温冷却显著降低了大米产量,对全球粮食安全构成威胁.
- 以前的研究集中在生理变化和编码基因上,忽视了在低温压力下的竞争性内源性RNA (ceRNA) 机制.
研究的目的:
- 为了研究低温压力下的大米中ceRNA机制.
- 确定关键的基因和参与抗氧化剂应对寒冷压力的调节网络.
主要方法:
- 综合抗氧化剂生理指数与全转录组数据.
- 使用权重基因共同表达网络分析 (WGCNA) 来识别与抗氧化酶相关的基因模块.
- 使用枢纽基因和丰富的代谢途径基因构建了ceRNA网络.
主要成果:
- 鉴定出与超氧化物脱氧化酶 (SOD) 和过氧化酶 (POD) 高相关的基因模块.
- 确定了关键的枢纽基因,包括UDP-glucosyltransferase,sessquiterpene synthase,indole-3-glycerophosphatase,WRKY转录因子,血膜 ATPase和类似受体的激酶.
- 构建了三个ceRNA网络,包括七个长非编码RNA,四个微RNA和三个信使RNA.
- 证实WRKY转录因子*OsWRKY61*具有负面的调节功能.
结论:
- 一个新的ceRNA调节网络参与了大米对低温压力的反应.
- 特定的长非编码RNA,微RNA和信使RNA在调解抗氧化剂防御机制中起着至关重要的作用.
- *OsWRKY61* 在这种寒冷应激反应途径中起到负调节作用.
更多相关视频
09:43Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
Published on: January 3, 2025
2.4K
08:39Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress
Published on: October 11, 2024
1.6K
相关概念视频
02:39lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
02:24Ribosome Profiling
3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
02:15Experimental RNAi
6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
01:20Types of RNA
5.8K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...
5.8K
02:45Responses to Heat and Cold Stress
13.5K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.5K
02:51mRNA Stability and Gene Expression
5.6K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
5.6K