维斯法在怀孕早期影响猪质细胞的转录组
Grzegorz Kopij1, Marta Kiezun1, Kamil Dobrzyn1
1Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn, Poland.
International journal of molecular sciences
|February 24, 2024
概括
维斯法丁/NAMPT (VIS) 显著影响猪白细胞基因表达和拼接,这对于维持妊娠至关重要. 这种荷尔蒙影响血管生成和类固醇生成等关键过程,对于成功的植入至关重要.
科学领域:
- 生殖生物学 生殖生物学
- 分子内分泌学分子内分泌学
- 基因组学就是基因组学.
背景情况:
- 维斯法/NAMPT (VIS) 是一种具有多种作用的激素,包括生殖和怀孕中的作用.
- 以前的研究表明,VIS会影响猪的垂体和卵巢功能.
研究的目的:
- 为了研究VIS对猪白细胞的全球转录组的影响.
- 识别受VIS.影响的差异表达基因 (DEG),长非编码RNA (DEL) 和替代拼接事件 (DAS).
主要方法:
- 在植入期内,猪的白色细胞被用VIS (100 ng/mL) 治疗.
- 使用RNA测序 (Illumina NovaSeq 6000) 来分析转录基因变化.
- 进行了差异基因表达,长非编码RNA和替代拼接分析.
主要成果:
- 确定了170个DEG (99个上调,71个下调) 和40个DEL (3个已知,37个新型).
- 检测到169个不同的替代拼接事件.
- 维斯显著影响了白细胞转录组和结合组.
结论:
- 维斯在调节猪白色细胞的转录体和结合体中发挥着重要作用.
- 这些变化会影响植入和维持怀孕的关键过程,包括血管生成,类固醇生成,炎症和细胞发育.
关键词:
在RNA-Seqq.这是一种阿迪波基因 (adipokine).替代性拼接是一种替代性的拼接.血管新生的产生.黄体体 (Corpus luteum) 是一个体.不同表达基因的差异性表达基因信号通道是指信号通道.更多相关视频
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