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探索多组织替代分离和骨肌肉代谢调节在肥胖和瘦类型猪

Wei Wang^1,2, Wangchang Li^2,3,4, Weiwei Liu^2,3,4

¹Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China.

|February 24, 2024

概括

替代拼接 (AS) 显著多样化了猪的转录组. 这项研究在9个组织中分析了AS,确定了成千上万的事件,特别是跳过的外因子,并突出了葡萄糖激素对品种差异的信号传递中的关键基因.

科学领域:

基因组学和分子生物学
转录学和转录后监管法规

背景情况:

替代拼接 (AS) 是一个关键的转录后调节机制.
AS产生转录组和蛋白质组的多样性,影响生物功能.
了解AS差异对于畜牧养殖和生理学至关重要.

研究的目的:

在杜罗克 (瘦) 和卢chuan (肥胖) 猪中全面描述替代拼接事件.
识别与表型差异相关的差异性AS的基因.
探索AS在不同猪组织中的功能影响.

主要方法:

在两个猪品种的9个组织中进行了全面的AS分析.
识别和量化AS事件,按类型分类 (例如,跳过的exon).
功能性丰富分析和与差异表达基因 (DEGs) 的重叠分析.

主要成果:

在14393个基因中确定了94990个AS事件.
跳过的exon (SE) 事件占所有识别的AS事件的80%.
具有广泛AS (>10事件) 的基因与组织特异性功能有关.
在心脏和骨肌肉中发现的差异替代拼接基因 (DSG) 和DEG之间的重叠最高.
在葡萄糖信号通路中验证了三个关键基因 (PYGM,MAPK11,CAMK2B),在品种之间存在显著的AS差异.

结论:

提供了关于AS调节及其在组织生理学中的作用的新见解.
突出了AS作为瘦猪和肥猪之间的表型差异的分子基础.
识别的AS签名对于改善养猪策略有价值.

关键词:

替代性拼接是一种替代性的拼接.多个组织的多个组织.肥胖和瘦猪类型的猪.骨肌肉的新陈代谢转录组 (transcriptome) 是一个转录组.

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