化合物会以不同的方式影响皮肤纤维细胞中的细胞质/二硫化物状态,并通过与细胞外基质相互作用来改善细胞迁移
Christine Kreindl1, Sandra A Soto-Alarcón2, Miltha Hidalgo1
1Laboratory for Research in Functional Nutrition, Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile, Santiago 7830490, Chile.
Antioxidants (Basel, Switzerland)
|February 24, 2024
概括
补充剂通过增强纤维细胞中的细胞外基质生产,改善了高葡萄糖条件下的伤口愈合. 具体来说,se-cysteine增强了氧化敏感性和二硫化键的减少,有助于内皮细胞的迁移.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 伤口治愈研究研究 伤口治愈研究
背景情况:
- 糖尿病会影响伤口愈合,通常与纤维细胞功能障碍和细胞外基质 (ECM) 生产受损有关.
- 纤维细胞回氧稳定对于ECM合成至关重要,包括原蛋白,影响伤口修复.
- 了解在纤维细胞氧化还原平衡中的作用,可能为糖尿病伤口并发症提供治疗策略.
研究的目的:
- 研究无机和有机化合物对纤维细胞ECM分泌和正常和高葡萄糖条件下的氧化还原状态的影响.
- 通过使用HyPer生物传感器,评估对纤维细胞氧化还原恒温的影响.
- 评估处理对ECM质量和内皮细胞迁移在伤口愈合模型中的功能影响.
主要方法:
- 在正常 (5毫米) 和高 (25毫米) 的葡萄糖介质中培养人类皮肤纤维细胞,用胺,Se-cysteine或Se-methionine进行10天的培养.
- 使用扫描电子显微镜 (SEM) 来分析分泌的ECM中的超结构变化.
- 使用HyPer生物传感器测量纤维细胞的氧化还原状态和对刺激的实时反应.
主要成果:
- 补充剂使纤维细胞的氧化还原平衡转向更氧化状态,不论的形式如何.
- 在高葡萄糖纤维细胞中,se-cysteine独特地增强了氧化刺激的敏感性和二硫化键的减少.
- 虽然没有发现氧化还原概况和ECM超结构之间的直接相关性,但改善了ECM质量,在伤口愈合试验中增强了内皮细胞迁移.
结论:
- 化合物,特别是Se-cysteine,显示出减轻高葡萄糖诱导对纤维细胞功能和ECM产生有害影响的潜力.
- 能够调节氧化还原平衡并提高ECM质量的能力,为增强糖尿病患者的伤口愈合提供了一个有前途的途径.
- 需要进一步研究对抗糖化诱导的原体损伤的保护机制.
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