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传染性呼吸道病原体对呼吸生物标志物的影响
Nele Kemnitz1, Patricia Fuchs1, Rasmus Remy1
1Rostock Medical Breath Research Analytics and Technologies (ROMBAT), Department of Anaesthesiology, Intensive Care Medicine and Pain Therapy, University Medicine Rostock, 18057 Rostock, Germany.
Antioxidants (Basel, Switzerland)
|February 24, 2024
概括
呼出的挥发性有机化合物 (VOC) 可以反映实时感染状态,但广泛的个体变化意味着只有显著的VOC变化表明疾病. 这项研究分析了真实环境中的呼吸形状.
科学领域:
- 生物化学 生物化学
- 微生物学 微生物学
- 分析化学 分析化学
背景情况:
- 呼出的挥发性有机化合物 (VOC) 提供了对宿主反应和病理生理学的非侵入性见解.
- 呼吸道感染的代谢分析对早期病原体检测和疾病监测至关重要.
研究的目的:
- 在现实世界查环境中调查常见呼吸道病原体对呼出的VOC概况的影响.
- 评估VOCs的诊断潜力,以识别流感和其他呼吸道疾病等感染.
主要方法:
- 质子转移反应飞行时间质谱法 (PTR-ToF-MS) 分析了479人的呼吸样本.
- 多重PCR识别了呼吸道病原体 (例如,Hemophilus influenzae,Streptococcus pneumoniae,鼻病毒).
- 挥发性有机化合物 (VOC) 概况与PCR结果和临床症状相关.
主要成果:
- 呼出的VOC档案被Haemophilus influenzae,Streptococcus pneumoniae和Rhinovirus的存在所改变.
- 观察到内源子,短链脂肪酸,有机硫,化物和烯的变化.
- 很少有VOC度变化超过生理变化,突出显著的人际差异.
结论:
- 在现实查条件下,VOC概况存在广泛的个体间差异.
- 没有发现感染的独特VOC标志物;只有与基线的显著偏差是指示性的.
- 挥发性有机化合物分析显示了感染检测的潜力,但需要仔细考虑个体变异性.
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