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描述中性粒细胞丰富的三阴性乳腺癌的炎症概况

Fatma Al Qutami¹, Walaa AlHalabi¹, Aswathy Vijayakumar¹

¹Department of Medicine, Mohammed Bin Rashid University of Medicine and Health Sciences, Dubai P.O. Box 505055, United Arab Emirates.

|February 24, 2024

概括

乳腺癌细胞可以损害中性粒细胞,促进NETosis和炎症. 这种相互作用可能有助于攻击性瘤,如三阴性乳腺癌 (TNBC) 的进展.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

三重阴性乳腺癌 (TNBC) 与中性粒细胞透率的增加,预后不佳和免疫疗法的有效性降低有关.
了解中性粒细胞和乳腺癌细胞之间的相互作用对于开发新疗法至关重要.

研究的目的:

研究中性粒细胞对转移性TNBC (MDA-MB-231) 与转移性较小的MCF-7乳腺癌细胞系的双向作用.
探索乳腺癌细胞如何影响中性粒细胞的行为,包括活力,NETosis和炎症标志物表达.

主要方法:

使用了类似中性粒细胞的HL60细胞和乳腺癌细胞系 (MDA-MB-231,MCF-7).
评估了中性粒细胞活力,NETosis (核酸染色),炎症标记物表达 (流细胞计) 和迁移能力 (transwell测定).
从乳腺癌细胞中分析条件介质 (CM),以评估它们对中性粒细胞的影响.

主要成果:

乳腺癌细胞及其CM降低了中性粒细胞的生存能力.
与MCF-7 CM相比,MDA-MB-231 CM在分化的HL60细胞中诱导了更高水平的细胞应激和NETosis.
MDA-MB-231 CM促进了中性粒细胞炎症表型,包括增加粘附性,脱粒化,CD54表达和迁移能力.

结论:

乳腺癌,特别是TNBC,可以通过NETosis和补充激活利用中性粒细胞.
乳腺癌细胞和中性粒细胞之间的交叉声代表了推动瘤进展的潜在机制.
针对这种中性粒细胞与乳腺癌相互作用,可以提供新的治疗策略.

关键词:

网症 (NETosis) 是一种疾病.美国TNBC电视台他们是中性恋者.三重阴性乳腺癌是什么

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