16S rRNA下一代测序可能对检查自发细菌炎疑似病例不有用
Chan Jin Yang1, Ju Sun Song2, Jeong-Ju Yoo1
1Department of Internal Medicine, Soonchunhyang University Bucheon Hospital, Bucheon 14584, Republic of Korea.
Medicina (Kaunas, Lithuania)
|February 24, 2024
概括
下一代测序 (NGS) 对炎液的测序没有改善细菌检测率超过传统培养,即使在怀疑自发性细菌周周炎 (SBP). 浅石中的细菌生物量较低,限制了这两种方法的灵敏度.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 医学诊断 医学诊断 医学诊断
背景情况:
- ,通常与肝硬化有关,对细菌感染有诊断困难.
- 对于炎感染的传统诊断方法有局限性.
- 像16S rDNA下一代测序 (NGS) 这样的先进技术正在探索低生物质流体.
研究的目的:
- 为了比较16S rDNA NGS的检测灵敏度与常规的瘤培养.
- 为了调查腹腔中微生物群的存在.
- 为了确定腹腔是否无菌.
主要方法:
- 招募了十名疑似自发细菌性腹膜炎 (SBP) 的患者.
- 阿西特斯样本经历了传统培养和16S核糖体RNA基因放大和测序.
- 测序结果与正控和负控进行了比较.
主要成果:
- 离心后,从石样本中的细菌颗粒是最小的或不存在的.
- 与负对照相比,16S安普利康测序库显示了最小或低强度的目标产品.
- 序列分析揭示了与负对照相似的微生物组成,患者样本中没有发现独特的细菌.
结论:
- 腹腔腔和骨有较低的细菌生物量,即使在SBP的情况下.
- 16S rRNA基因测序显示,在流体中,阳性率非常低.
- 与传统的培养方法相比,NGS并不能显著提高阳性检测率,用于炎感染.
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