一个细分的Kindia虫病毒基因组的未翻译区域高度保存,并包含病毒复制的多个调节元素
Anastasia A Tsishevskaya1,2, Daria A Alkhireenko1,3, Roman B Bayandin1
1State Research Center of Virology and Biotechnology «Vector», 630559 Kol'tsovo, Russia.
Microorganisms
|February 24, 2024
概括
这项研究揭示了金迪亚病毒 (KITV) 的未翻译区域 (UTR) 中的关键调控元素,这是一个Jingmenvirus. 这些发现表明病毒复制中的功能作用,并突出了与orthoflaviviruses的进化联系.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 京门病毒 (JMVs) 是细分的,在全球范围内发现的传播的RNA病毒.
- 肯迪亚病毒 (KITV) 是JMV组的一员,对其基因组调节元素的理解有限.
- 病毒基因组的未翻译区域 (UTR) 在复制和调节中起着至关重要的作用.
研究的目的:
- 为了获得KITV分离物的全基因组序列.
- 模拟和功能地注释JMV和KITV的5'和3'UTR的二次结构.
- 在KITV UTR中识别监管元素并评估它们的进化意义.
主要方法:
- 两个KITV分离物的全基因组测序.
- 病毒UTR二次结构的生物信息建模和功能注释.
- 在KITV和其他JMV中对UTR主题和结构进行比较分析.
主要成果:
- 鉴定了保存的基因,包括多基化3' UTR,5'和3' DAR基因,5'-CACAG-3'序列和La蛋白结合点.
- 发现了MSI1蛋白相互作用的多个UAG位点和segment 2 UTRs内的同源序列.
- 在2段的UTR中特征独特的结构,包括Y-3结构和由于删除的变化,以及伪结和保存的发针序列 (例如,5'-CAAGUG-3').
结论:
- 在KITV UTR中识别出了具有orthoflaviviruses特征的新型调控元素.
- 这些元素可能在JMV复制中发挥功能作用.
- 这一发现表明,正方体状病毒和细分状病毒之间存在进化上的相似性.
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