生物控制通过温度和细菌的控制而改变
Alejandra Larach1,2,3, Paulina Vega-Celedón1,2, Diyanira Castillo-Novales1,2,3
1Escuela de Agronomía, Facultad de Ciencias Agronómicas y de los Alimentos, Pontificia Universidad Católica de Valparaíso, San Francisco s/n La Palma, Quillota 2260000, Chile.
Microorganisms
|February 24, 2024
概括
智利的PGPB和内菌对控制葡萄树干疾病,如Diplodia dieback,显示出有前途. 生物控制在低温 (8°C) 时最有效,这表明了现场应用的潜力.
科学领域:
- 植物病理学 植物病理学
- 微生物学 微生物学
- 农业科学 农业科学
背景情况:
- 葡萄树干疾病 (GTD) 对全球葡萄园构成重大威胁,影响葡萄酒生产.
- 排列 (Diplodia seriata) 是一个关键的病原体,它是致死菌 (Botryosphaeria dieback) 的致病原体,这是一个影响葡萄树的流行GTD.
- 当前的生物控制研究经常忽视温度变化,这是病原体感染周期的关键因素.
研究的目的:
- 评估智利植物生长促进细菌 (PGPB) 和葡萄藤内生菌对Diplodia seriata的生物控制疗效.
- 评估不同温度 (8°C,22°C,35°C) 对体外和体内生物控制活动的影响.
- 为了确定有效的细菌菌株和条件来管理D. seriata在葡萄种植.
主要方法:
- 在体外对抗性试验中,使用阿加塞扩散和双板方法,使用三种D. seriata分离物进行了对抗性试验.
- 对三种细菌菌株 (Pseudomonas sp. GcR15a, 伪omonas sp. 这种类型. 在AMCR2b,Rhodococcus sp. 的情况下. PU4) 在三种温度下.
- 在Cabernet Sauvignon切片上进行体内疗效测试,以验证体外发现.
主要成果:
- 在8°C的阿加插头扩散方法产生了最高的抑制率,与Rhodococcus sp. 在PU4 (65%) 和Pseudomonas sp.中使用. GcR15a (57%) 显示显著的控制.
- 在22°C时,只有Pseudomonas菌株表现出对D. seriata的生物控制活性.
- 在35°C时,一种Pseudomonas菌株实现了38%的控制,相当于tebuconazole (33%),而Rhodococcus sp. 显示了30%的控制. 在体内测试证实了对两种D. seriata分离物的生物控制,其中一种分离物表现出耐药性.
结论:
- 细菌生物控制剂,特别是Pseudomonas和Rhodococcus物种,对Diplodia seriata具有显著的潜力.
- 低温 (8°C) 提高了某些生物控制菌株的疗效,与GTD冬季感染期保持一致.
- 这些发现支持开发特定于温度的生物控制策略,用于管理智利葡萄园中的葡萄树干疾病.
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