非洲猪瘟病毒I267L是一种与出血相关的基因,基于转录组分析
Yuan Wen1,2, Xianghan Duan1,2, Jingjing Ren1,2
1State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730000, China.
Microorganisms
|February 24, 2024
概括
非洲猪瘟病毒基因I267L与疾病严重程度有关. 在病毒中删除I267L部分恢复了F3基因表达,这表明I267L通过抑制F3和影响凝血来促进非洲猪瘟 (ASF).
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 遗传学 遗传学 是一个
背景情况:
- 非洲猪瘟 (ASF) 是一种高度致命的病毒性疾病,影响着全球猪业.
- 非洲猪瘟病毒 (ASFV) 具有很大的基因组,了解个体基因功能对于控制至关重要.
- 在猪中,ASF感染会导致高烧,炎症,出血和死亡.
研究的目的:
- 为了研究ASFV基因I267L在病毒病原性中的功能.
- 了解I267L在ASFV感染期间宿主免疫和凝血反应中的作用.
主要方法:
- 感染野生型ASFV和I267L删除菌株 (∆I267L) 的猪膜巨细胞 (PAMs) 的转录组序列 (RNA-seq).
- 定量实时PCR (RT-qPCR) 用于验证RNA-seq发现.
- 分析与炎症和凝血通路相关的基因表达.
主要成果:
- 炎症和凝血中的关键环节F3基因的表达显著改变.
- 凯格 (KEGG) 分析显示,补充和凝血级联受到ASFV感染的影响.
- 删除I267L部分扭转了ASFV通过TNF-α抑制F3诱导的情况.
结论:
- ASFV基因I267L与病原性相关,通过抑制F3表达来调节ASF中的出血.
- 在ASFV感染期间,I267L在调节宿主的炎症和凝血反应中发挥作用.
- 这项研究提供了关于ASFV分子机制和疾病控制的潜在目标的见解.
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