登革热病毒的特征 巴拉圭的4例病例,2019-2020年
Alejandra Rojas1, John Shen2, Fátima Cardozo1,3
1Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, San Lorenzo 111241, Paraguay.
Viruses
|February 24, 2024
概括
第4型登革热病毒在2019-2020年导致巴拉圭最大的爆发. 遗传学分析揭示了在2018年之前在巴拉圭和巴西流通的菌株,强调需要持续的基因组监测.
科学领域:
- * 病毒学和流行病学
- * 对阿尔博病毒的基因组监测
背景情况:
- *巴拉圭在2019-2020年经历了最大的登革热病毒 (DENV) 爆发,主要是由DENV4型驱动的.
- *了解这次疫情的流行病学特征和起源对于公共卫生干预至关重要.
研究的目的:
- *与其他急性发烧性疾病相关的登革热病例的特征.
- *使用遗传学分析来确定巴拉圭DENV-4疫情的起源.
- * 评估快速登革热非结构蛋白1 (NS1) 试验的诊断性能.
主要方法:
- *从2019年1月到2020年3月,招募患有急性疾病 (≤7天) 的个人.
- *使用非结构蛋白1 (NS1) 抗原和实时RT-PCR检测病毒RNA的DENV诊断测试.
- * 62个DENV-4阳性样本的全基因组测序和随后的遗传学分析.
主要成果:
- * 在799名参与者中,253人患有登革热 (5.5%严重),在94.1%的登革热病例中检测到DENV-4.
- *与RT-PCR相比,NS1快速测试显示了52.5%的灵敏度和96.5%的特异性.
- * 遗传学分析将DENV-4序列放置在基因型II中的两个分类中,与2018年巴拉圭和2013年巴西菌株密切相关.
结论:
- * DENV-4 能够引起严重病例的重大爆发.
- *目前为登革热NS1的快速诊断测试具有有限的灵敏度.
- *2019-2020年DENV-4疫情株可能在2018年之前在巴拉圭和巴西传播,这强调了持续基因组监测的必要性.
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