应用高通量测序用于葡萄藤中综合性病毒组概况的应用在伊朗显示黄色的葡萄
Zahra Gholampour1, Mohammad Zakiaghl1, Elisa Asquini2
1Department of Plant Pathology, College of Agriculture, Ferdowsi University of Mashhad, Mashhad 9177948978, Iran.
Viruses
|February 24, 2024
概括
这项研究在伊朗葡萄树中发现了13种病毒和病毒,包括新的葡萄红斑病毒和葡萄卫星病毒. 为了可靠地检测葡萄藤病毒,建议使用14%的重建值.
科学领域:
- 植物病毒学 植物病毒学
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 葡萄藤 (Vitis vinifera) 拥有复杂的微生物群,包括病毒和病毒,影响作物产量和质量.
- 了解葡萄藤病毒组对于疾病管理,隔离和认证计划至关重要.
- 之前在伊朗进行的研究还没有全面分析葡萄葡萄病毒和病毒的整个谱.
研究的目的:
- 对伊朗五种葡萄品种的病毒组进行全面分析.
- 从小RNA测序 (sRNA-seq) 数据进行病毒检测和基因组重建的不同生物信息方法的比较.
- 识别新型病毒和病毒病毒检测并建立可靠的检测值.
主要方法:
- 小RNA (sRNA) 库是从五种伊朗葡萄品种的花皮组织中准备的.
- 用两种不同的生物信息学方法分析了sRNAome数据,以评估病毒组成和丰度.
- 评估了病毒基因组重建和检测值.
主要成果:
- 一个复杂的葡萄藤病毒体被描述,在五种品种中发现了十三种病毒和病毒体.
- 葡萄红斑病毒和葡萄卫星病毒首次在伊朗葡萄园中检测到.
- 葡萄藤叶卷相关病毒1 (GLRaV1) 和葡萄藤风叶病毒 (GFLV) 主导,种类之间流行率有所不同.
- 混合感染,如GLRaV1/GYSVd1和GFLV/GYSVd1,与特定的葡萄树症状有关,如黄色和静脉带状.
- 14%的完整基因组重建的门被提出为葡萄藤病毒检测的可靠值.
结论:
- 伊朗葡萄藤病毒群是多样化和复杂的,品种之间存在显著的差异.
- 生物信息对齐器的选择显著影响病毒检测和从sRNA-seq数据的基因组重建.
- 拟议的14%的重建值为在诊断和认证环境中识别葡萄藤病毒提供了可靠的指标.
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