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Updated: Aug 3, 2026

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
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Published on: June 16, 2017

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基于全基因组CRISPR淘汰屏幕的牛疹病毒1型候选宿主细胞进入因子的验证

Wenfang Spring Tan1, Enguang Rong1, Inga Dry1

  • 1Division of Infection and Immunity, the Roslin Institute, Easter Bush Campus, University of Edinburgh, Edinburgh EH259RG, UK.

Viruses
|February 24, 2024
PubMed
概括

研究人员确定了与脊髓灰质炎病毒受体相关的蛋白质 (PVRL2) 和脊髓灰质炎病毒受体 (PVR) 是牛疹病毒1型 (BoHV-1) 的关键入口受体. 这项研究还突出了肝酸硫酸蛋白质糖 (HSPG) 在病毒进入和复制中的作用.

关键词:
这种病毒是BoHV-1病毒.复杂的COG复合体可以这就是CRISPR/Cas9的作用.戈尔吉仪器是一个巨大的装置.细胞的入口是细胞的入口.肝硫酸盐 肝硫酸盐 是一种这些受体是受体受体.

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this defense.
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CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...