转录因子ThDOF8与一种新型的cis元素结合,并调解Tamarix hispida中对盐应激的分子反应
Peilong Wang1,2, Danni Wang1, Yongxi Li1
1State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), Harbin 150040, China.
Journal of experimental botany
|February 24, 2024
概括
一个新发现的基因,ThDOF8,通过提高林和抗氧化酶水平,增强了Tamarix hispida的盐耐受性. 这种用一根手指转录因子结合DNA的方法针对特定的基因,为改善植物盐分耐受性提供了一个新的模型.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物压力生理学 植物压力生理学
- 遗传学和基因组学 遗传学和基因组学
背景情况:
- 盐应激是限制植物生长的主要非生物因素.
- 了解像Tamarix hispida这样的半植物的盐耐受机制至关重要.
- 一指DNA结合 (DOF) 转录因子在盐应激反应中的作用在T. hispida.中基本上是未知的.
研究的目的:
- 确定和表征一种新的DOF基因 (ThDOF8),该基因与T. hispida.中的盐应激反应有关.
- 阐明ThDOF8在调节盐分耐受性的调节机制.
- 为了识别ThDOF8.8的直接标基因.
主要方法:
- 在盐应力下对ThDOF8的基因克隆和表达分析.
- 在T. hispida和Arabidopsis.中,ThDOF8的过度表达是暂时的和稳定的.
- 酵母单杂交 (Y1H),电泳运动转移试验 (EMSA) 和染色体免疫沉 (ChIP-qPCR) 用于识别DNA结合和向基因.
- 测量林含量和抗氧化酶活性 (SOD,POD).
主要成果:
- 在T. hispida. 中,ThDOF8表达是由盐应激诱导的.
- 过度表达ThDOF8通过增加蛋白水平和抗氧化酶活性 (SOD,POD) 来提高盐分耐受性.
- ThDOF8直接与目标基因的促进子区域的cis元素TGCG结合,包括CRK10,CRK26,GAD41和GAD42.
- 过度表达ThCRK10也会导致盐分耐受性.
结论:
- 在T. hispida.中,ThDOF8在调节盐分耐受性方面发挥着重要作用.
- 提出了一个新的调控模型,其中ThDOF8与CRK10促进体中的TGCG元素结合,以增强盐分耐受性.
- 这项研究提供了对DOF转录因子功能的见解,并通过分子育种确定了改善植物盐耐受性的潜在基因.
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