用高性能液态染色学量化纳米乳液中的单类 (p-Cymene和Myrcene) 的分析方法
Jonatas Lobato Duarte1, Leonardo Delello Di Filippo1, Alberto Gomes Tavares1
1São Paulo State University (UNESP), Department of Drugs and Medicines, School of Pharmaceutical Sciences, Rodovia Araraquara Jaú, Km 01 - s/n - Campos Ville Araraquara, São Paulo, 14800-903, Brazil.
Journal of AOAC International
|February 24, 2024
概括
一种新的HPLC方法准确地量化了纳米乳液中的明和p-cymene. 这种经过验证的技术确保了在先进的药物输送系统中对这些单类药物的质量控制.
科学领域:
- 分析化学 分析化学
- 制药科学 制药科学
- 纳米技术 纳米技术
背景情况:
- 米尔和p-cymene是具有有限溶解性的挥发性单类.
- 纳米乳液为活性成分提供了更好的生物可用性和稳定性.
- 这些单类药物的精确处理和配方存在挑战.
研究的目的:
- 开发和验证一种高性能液体染色学 (HPLC) 方法.
- 在纳米乳液配方中具体量化p-cymene和myrcene.
主要方法:
- 使用了一个Phenomenex® SynergiTM Fusion-RP柱.
- 采用同位素化与乙酸-水移动相 (60:40,v/v).
- 验证了方法的特异性,线性,准确性,精度,稳定性和选择性.
主要成果:
- 实现了高准确度和精度,相关系数为0.999.
- 报告的相对标准偏差 (RSD) 值低于2%.
- 证明方法适用于质量控制和未来的研究.
结论:
- 经过验证的HPLC方法可用于量化纳米乳液中的单类.
- 该方法可以作为纳米乳液配方的可靠质量控制工具.
- 证实了确保一致的制定和指导未来研究的潜力.
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