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Updated: Oct 2, 2026

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization
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一种重组阿斯伯吉卢斯尼格尔GZUF36脂酶的特性,该脂酶通过离子液修饰策略被固定

Shuqi Xing1,2,3, Jia Long2,3, Wei Xie2,3

  • 1Center for Research and Development of Fine Chemicals, Guizhou University, Guiyang, 550025, People's Republic of China.

Applied microbiology and biotechnology
|February 24, 2024
PubMed
概括

这项研究成功地将Aspergillus niger脂酶 (PEXANL1) 固定在磁性纳米粒子 (Fe3O4@SiO2@ILs) 上. 由此产生的纳米生物催化剂显示出增强的活性,稳定性和可用于工业应用的可重复使用性.

关键词:
固定化 固定化 固定化离子液体改性磁性纳米材料利帕斯 (Lipase) 是一种脂肪酶.多点吸附的多点吸附方式.可重复使用性 可重复使用性

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OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy08:34

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Production of Pharmaceuticals01:30

Production of Pharmaceuticals

Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...