在Ustilago maydis中增强的氧化应激抵抗力及其对病毒毒性的影响
Jorge Cuamatzi-Flores1,2, Maritrini Colón-González3, Fernanda Requena-Romo3,4
1Unit for Basic and Applied Microbiology, Faculty of Natural Sciences, Autonomous University of Queretaro, 76230, Querétaro, México. jcuamatzi@liigh.unam.mx.
概括
在玉米污泥真菌Ustilago maydis中对过氧化 (H2O2) 的增强耐药性并没有增加毒性. 相反,通过适应或催化酶过度表达,增加的H2O2耐药性降低了真菌感染植物的能力.
科学领域:
- 植物病理学 植物病理学
- 菌类学 菌类学是指菌类学.
- 氧化压力生物学 氧化压力生物学
背景情况:
- 乌斯蒂拉戈梅迪斯通过抑制宿主防御,包括氧化爆发,导致玉米污垢.
- 虽然研究了U. maydis对氧化应激的反应,但增强的氧化应激抵抗对其毒性的影响不太清楚.
研究的目的:
- 调查增强的过氧化 (H2O2) 耐药性对Ustilago maydis.病毒毒性的影响.
主要方法:
- 乌梅迪斯SG200通过不断升级的冲击来适应高H2O2度.
- 基因分析发现了适应菌株的突变,包括一个catalase基因放大.
- catalase被过度表达以评估其在H2O2耐药性和毒性中的作用.
主要成果:
- 一种适应的U. maydis菌株对60mM H2O2. 2产生了耐药性.
- 基因分析显示,在适应的菌株中,催化酶基因的大幅放大.
- 适应和过度表达酶的两种菌株都表现出减少的毒性.
结论:
- 在U. maydis中增强的H2O2耐药性,通过适应或催化酶过度表达,可以降低毒性.
- 在感染期间,真菌对氧化应激的自然反应似乎是最佳的.
- 增加的H2O2耐药性不会增强U.maydis的毒性,揭示了复杂的相互作用.
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