用海洋细菌Enterobacter tabaci RAU2C进行棕色海藻的固体发酵,以生产酸酶
Ramya Petchimuthu1, Subharaga Venkatesh1, Suriyalakshmi Kannan1
1Department of Biotechnology, Kalasalingam Academy of Research and Education, Krishnankoil-626126, Tamilnadu, India.
Folia microbiologica
|February 24, 2024
概括
这项研究通过固态发酵 (SSF) 优化了使用海藻生物质的酸盐酶生产. 该过程产生了显著的酶活性,证明了海藻.
科学领域:
- 生物技术和工业微生物学
- 酶技术 酶技术是一种
- 海洋生物技术 海洋生物技术
背景情况:
- 酸酸酶是有价值的生物催化剂,在生物燃料和生物活性寡糖生产中具有应用.
- 海藻生物质为酶生产提供了可持续且具有成本效益的基质.
- 固态发酵 (SSF) 在酶产量和能源效率方面比浸泡发酵具有优势.
研究的目的:
- 使用海洋细菌优化棕色海藻的生物处理,以生产酸酶.
- 通过SSF研究使用Sargassum swartzii作为低成本基质,通过SSF进行酸酶.
- 确定最佳的过程参数,以提高酸盐酶产量和活性.
主要方法:
- 使用了一种海洋细菌,Enterobacter tabaci RAU2C,用于酸酶的生产.
- 在固态发酵 (SSF) 中采用 Sargassum swartzii,优化了注射液,潜伏期和水分含量.
- 评估了缓冲器,pH值和温度对酶活性的影响.
主要成果:
- 在静态SSF条件下,在4天后达到33.56U/mL的酸酶,水分为75%.
- 证明酶活性增加,含水量在60%至75%之间.
- 在pH 7和37°C使用酸盐缓冲器时,确定了最大酶活性的最佳条件.
- 初步分析表明,残留生物质可以用作生物肥料.
结论:
- 通过SSF通过海藻生物质成功证明了使用海藻生物质生产酸酶.
- 建立了最大限度地提高酸盐酶产量和活性的最佳条件.
- 这项研究是第一个关于使用海藻生物质作为SSF的酸盐酶生产基质的研究报告.
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