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在高质量和低质量的甲期II卵子细胞中,形和体积不同

Monika Fluks¹, Robert Milewski², Szymon Tamborski³

¹Department of Embryology, Institute of Developmental Biology and Biomedical Sciences, Faculty of Biology, University of Warsaw, Warsaw, Poland.

Reproduction (Cambridge, England)

|February 24, 2024

概括

光学连贯显微镜 (OCM) 非侵入性地评估第二阶段转移期卵细胞螺旋质量. 将OCM螺旋分析与胚胎形态动力学结合起来,可以改善卵细胞和胚胎选择以获得IVF成功.

科学领域:

生殖生物学生殖生物学
生物医学成像学生物医学成像学
在体外受精 (IVF)

背景情况:

卵细胞质量对于成功的胚胎发育和体外受精结果至关重要.
准确的卵细胞评估有助于明智的治疗决策和患者期望管理.
像子这样的细胞内结构与胚胎发育的成功有关.

研究的目的:

调查光学连贯显微镜 (OCM) 对于评估第二阶段 (MII) 蛋细胞质量的有用性.
为了确定MII线的定量OCM参数是否与胚胎发育和质量相关.
评估是否将OCM轴分析与形态动力学结合起来可以提高胚胎质量评估.

主要方法:

用小鼠模型对MII卵子细胞的OCM成像的应用.
非侵入性3D可视化和MII体积和形状的定量分析.
开发一个多变量线性回归模型,集成OCM数据和胚胎形态动力学.

主要成果:

与胚胎质量相关的OCM识别的MII子参数 (形状,体积),包括胚胎形成和 trofhectoderm/原始内皮的分化.
结合了OCM和形态动力学模型,显著改善了胚胎质量评估.
在植入前的发育过程中,OCM成像没有对细胞活力产生不利影响.

结论:

OCM提供了一种非侵入性的方法,用于对MII形态的定量评估.
基于OCM的螺旋分析,特别是与形态动力学相结合时,可以增强IVF中的卵细胞和胚胎选择.
这种方法有可能通过优化患者治疗策略来提高试管婴儿成功率.

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