简单的多信号校准利用流量分析系统
Vivian Maringolo1, Alexandre Z Carvalho1, Diogo L Rocha1
1Center on Natural and Human Sciences, Federal University of ABC, 09210-580, Santo André, SP, Brazil.
Talanta
|February 24, 2024
概括
新的多信号校准方法,在线多能量校准 (OMEC) 和多分散校准 (MDC),在基于流量的系统中提供准确和高效的分子光谱化学分析. 这些技术简化了样品的准备,并将矩阵效应降到最低,以便可靠地量化分析物.
科学领域:
- 分析化学 分析化学
- 谱化学分析 谱化学分析
- 基于流量的系统.
背景情况:
- 在分子光谱化学分析中,传统的校准方法可能受到矩阵效应和分析频率的限制.
- 多信号校准策略通过分析样品和标准混合物来提高准确性和效率提供了替代方案.
- 现有的多信号方法需要仔细考虑样本准备和潜在的矩阵干扰.
研究的目的:
- 开发和验证基于流量分析系统的两个新型多信号校准方法:在线多能校准 (OMEC) 和多分散校准 (MDC).
- 评估OMEC和MDC的可行性和适用性,以确定真实世界样本中的分析物.
- 将OMEC和MDC的性能与传统的外部校准 (EC) 和标准加法方法进行比较.
主要方法:
- 基于多个波长的光谱反应开发OMEC.
- 基于对样本分散概况的分析,开发了MDC.
- 这两种方法在以流量为基础的系统中应用,使用用标准和水稀释的样本溶液,用KMnO4,牛奶中的尿素和物盆喷雾剂进行测试.
主要成果:
- OMEC和MDC在连续和脉冲流程中证明了可行性,成功量化了KMnO4.
- 在牛奶和物小便器喷雾中测定尿素的光谱光度显示出很好的准确性,回收率从93%到101%不等.
- 用MDC (13 mg L-1) 和OMEC (16 mg L-1) 与EC (10 mg L-1) 相比,尿素的检测极限是可比的或改进的.
结论:
- 拟议的MDC和OMEC方法在以流量为基础的分流器内有效和高效地用于分析目的.
- 这些多信号校准策略在简单性和矩阵效应最小化方面具有优势.
- 该研究证实了OMEC和MDC在复杂样品中准确量化分析物的实际应用.
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