Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Homologous Recombination02:31

Homologous Recombination

50.5K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.5K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.8K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

The resolution revolution revisited.

IUCrJ·2026
Same author

Tonotopic specialization of MYO7A isoforms in auditory hair cells.

Nature communications·2026
Same author

DNA Assembly Templated by Chiral Nanotube Lattices: From Helix to Rings.

Journal of the American Chemical Society·2026
Same author

Structural determinants of endopilus assembly, stability and functional specificity in bacterial type II secretion.

bioRxiv : the preprint server for biology·2026
Same author

Supramolecular Assembly of Collagen-Mimetic Peptide D-Periodic Fibrils and Nanoassemblies.

Biomacromolecules·2026
Same author

Cryo-Structural Insights into Enzymatic Peptide Self-Assembly Driving Extrinsic Lytic Cell Death.

Journal of the American Chemical Society·2026
Same journal

Metabolic disruptions through a three-dimensional genomic lens.

Current opinion in structural biology·2026
Same journal

Collective variable design for biomolecular conformational dynamics.

Current opinion in structural biology·2026
Same journal

Polymer scaling in protein crowding: From dilute coils to semidilute meshes.

Current opinion in structural biology·2026
Same journal

Tuning the physicochemical properties of rationally designed protein-based biomolecular condensates.

Current opinion in structural biology·2026
Same journal

Editorial overview: Folding, binding and protein design.

Current opinion in structural biology·2026
Same journal

Macromolecular crowding reshapes the conformational landscapes of intrinsically disordered proteins: mechanisms, cellular contexts, and functional consequences.

Current opinion in structural biology·2026
查看所有相关文章

相关实验视频

Updated: Jul 2, 2025

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction
12:38

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Published on: August 9, 2011

17.5K

螺旋式重建,再次使用螺旋式重建.

Edward H Egelman1

  • 1Department of Biochemistry and Molecular Genetics, University of Virginia, 1340 Jefferson Park Avenue, Charlottesville, VA 22903, USA.

Current opinion in structural biology
|February 24, 2024
PubMed
概括
此摘要是机器生成的。

使用冷电子显微镜 (cryo-EM) 确定螺旋聚合物的正确对称性仍然具有挑战性. 目前的方法通常依赖于试错,突出了结构生物学中需要改进方法的需要.

更多相关视频

3D Planning and Printing of Patient Specific Implants for Reconstruction of Bony Defects
08:15

3D Planning and Printing of Patient Specific Implants for Reconstruction of Bony Defects

Published on: August 4, 2020

6.4K
Helical Organization of Blood Coagulation Factor VIII on Lipid Nanotubes
12:24

Helical Organization of Blood Coagulation Factor VIII on Lipid Nanotubes

Published on: June 3, 2014

12.3K

相关实验视频

Last Updated: Jul 2, 2025

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction
12:38

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Published on: August 9, 2011

17.5K
3D Planning and Printing of Patient Specific Implants for Reconstruction of Bony Defects
08:15

3D Planning and Printing of Patient Specific Implants for Reconstruction of Bony Defects

Published on: August 4, 2020

6.4K
Helical Organization of Blood Coagulation Factor VIII on Lipid Nanotubes
12:24

Helical Organization of Blood Coagulation Factor VIII on Lipid Nanotubes

Published on: June 3, 2014

12.3K

科学领域:

  • 结构生物学是结构生物学.
  • 生物物理学的生物物理.
  • 生物化学 生物化学

背景情况:

  • 螺旋型聚合物在生物系统中很常见,形成必要的蛋白质和核蛋白质复合体.
  • 低温电子显微镜 (cryo-EM) 技术的进步现在已经使这些组件能够实现近原子分辨率.
  • 确定这些螺旋结构的正确对称性是一个重大障碍.

研究的目的:

  • 本综述探讨了使用冷EM确定螺旋聚合物的对称性的困难.
  • 它强调了当前方法的局限性.

主要方法:

  • 该综述综合了现有的关于对螺旋聚合物应用的冷电磁技术的文献.
  • 它讨论了在对称性确定中遇到的挑战.

主要成果:

  • 对螺旋组件的准确对称性确定仍然是结构生物学中的一个复杂问题.
  • 目前的方法往往不够,需要进一步开发.

结论:

  • 缺少一种强大的,系统的方法来确定螺旋聚合物的对称性是一个关键的缺口.
  • 需要改进的策略来克服冷EM结构分析中的试错限制.