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相关概念视频

Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

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The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
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T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

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T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
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Crossover Experiments01:16

Crossover Experiments

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Crossover experiments, also called the repeated-measurements design, is a study design in which all experimental units are exposed to all treatments in different periods. Crossover experiments are generally used in psychology, the pharmaceutical industry, agriculture, and medicine.
Crossover designs are performed even with smaller sample sizes since the samples can act as their controls. These are better than simple randomized trials since patients are exposed to all the treatments.
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Desensitization and Tachyphylaxis01:20

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Tachyphylaxis is described as a rapid decrease in response to a drug after repeated or continuous administration of the same drug dose. It is a phenomenon where the body becomes less responsive to a particular substance or intervention over time, requiring higher doses or stronger interventions to achieve the same effect. It results from adaptive changes in the body's receptors, signaling pathways, or physiological processes that occur in response to prolonged exposure to a stimulus.
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Dose-Response Relationship: Overview01:03

Dose-Response Relationship: Overview

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Agonists can bind with and activate receptors, resulting in the formation of drug-receptor complexes. Once formed, these complexes catalyze many biochemical processes at the cellular level and subsequently induce a pharmacologic response. The degree of response is directly proportional to the fraction of activated receptors, which in turn, depends on the concentration of the drug at the receptor site as well as the sensitivity of the receptor. An increase in the administered dose contributes to...
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Longitudinal Research02:20

Longitudinal Research

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Sometimes we want to see how people change over time, as in studies of human development and lifespan. When we test the same group of individuals repeatedly over an extended period of time, we are conducting longitudinal research. Longitudinal research is a research design in which data-gathering is administered repeatedly over an extended period of time. For example, we may survey a group of individuals about their dietary habits at age 20, retest them a decade later at age 30, and then again...
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相关实验视频

Updated: Jul 2, 2025

Fear Incubation Using an Extended Fear-Conditioning Protocol for Rats
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克隆差异是对连续和长期治疗的可变反应的基础.

Dylan L Schaff1, Aria J Fasse2, Phoebe E White3

  • 1Department of Bioengineering, School of Engineering and Applied Sciences, University of Pennsylvania, Philadelphia, PA 19146, USA.

Cell systems
|February 24, 2024
PubMed
概括
此摘要是机器生成的。

癌细胞表现出多样化的基因表达,预测治疗耐药性. 克隆保持类似的状态,但在持续或新疗法下具有不同的命运,指导最佳治疗选择.

关键词:
条形码编码 条形码编码癌症系统生物学生物学克隆追踪是指克隆的追踪.药物耐药性 耐药性 药物耐药性这就是scRNA-seqq.

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Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER
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A Computerized Test Battery to Study Pharmacodynamic Effects on the Central Nervous System of Cholinergic Drugs in Early Phase Drug Development
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相关实验视频

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科学领域:

  • 在瘤学瘤学.
  • 基因组学就是基因组学.
  • 细胞生物学 细胞生物学

背景情况:

  • 癌细胞表现出显著的单细胞基因表达异质性,影响治疗耐药性.
  • 治疗干预可以加剧这种异质性,导致多样化的抗性细胞状态.
  • 在各种治疗压力下,这些耐药克隆的长期命运仍然不完全理解.

研究的目的:

  • 在长期和顺序治疗期间,研究癌症克隆中基因表达状态的稳定性.
  • 为了确定不同的耐药克隆是否对随后的治疗挑战表现出不同的反应.
  • 为了确定克隆生存和治疗向的预测基因表达标记物.

主要方法:

  • 使用单细胞RNA测序与DNA条形码结合使用.
  • 通过多个治疗周期追踪了耐药癌症克隆的进化.
  • 分析基因表达特征,以将状态与克隆命运相关联.

主要成果:

  • 同一个克隆中的细胞在重复治疗后保持一致的基因表达特征.
  • 个体克隆对二次治疗或延长的初始治疗表现出独特和不同的反应 (生长,存活,死亡).
  • 确定了与克隆生存相关的特定基因表达特征.

结论:

  • 癌症克隆异质性,虽然在克隆中稳定,但导致了明显的治疗脆弱性.
  • 了解克隆特异性反应对于开发有效的个性化癌症疗法至关重要.
  • 这项研究为选择针对攻击性耐药克隆的疗法提供了基础.